Lowered dietary phosphorus affects intestinal and renal gene expression to maintain mineral homeostasis with immunomodulatory implications in weaned piglets.
Lowered dietary phosphorus affects intestinal and renal gene expression to maintain mineral homeostasis with immunomodulatory implications in weaned piglets.
复制标题
DOI：
10.1186/s12864-018-4584-2
复制
发表时间：
2018-03-20
期刊：
影响因子：
4.4
通讯作者：
Wimmers K
中科院分区：
文献类型：
--
作者：
Just F;Oster M;Büsing K;Borgelt L;Murani E;Ponsuksili S;Wolf P;Wimmers K
在单胃动物中，磷(P)的平衡是通过调节肠道吸收、骨动员和肾脏排泄来维持的。由于磷是一种不可再生资源，由于农业和畜牧业普遍过度使用，短缺迫在眉睫。因此，在生猪生产中应提高磷效率。我们试图表征猪的Re−/吸收和排泄组织对不同钙磷比饲料的转录反应。断奶仔猪被分配到三组中的一组，饲喂不同可消化磷含量的日粮，为期五周。分析空肠、结肠和肾脏的基因表达谱。转录组分析表明，日粮磷摄入量减少影响了空肠和肾脏的基因表达，但不影响结肠的基因表达。空肠和肾脏中CYP24A1、CYP27A1、TRPM6、SPP1和VDR的mRNA丰度变化反映了矿物质动态平衡的调节。此外，在空肠中观察到与经典补体系统途径相关的转录本丰度降低。在肾脏中，移位的转录本涉及磷脂酶C、钙信号和NFAT信号，这可能具有免疫调节作用。我们的结果揭示了可变磷摄入量在肠道和肾脏组织中的局部转录后果。适应性反应是机体努力维持全身矿物质平衡的结果，同时调节局部组织部位的免疫功能。因此，必须仔细考虑偏离当前推荐的膳食磷供应量，因为对低磷饮食作出反应的内源性机制可能会影响重要的适应性免疫反应。本文的在线版本(10.1186/s12864-0184584-2)包含补充材料，可供授权用户使用。
In monogastric animals, phosphorus (P) homeostasis is maintained by regulating intestinal absorption, bone mobilization, and renal excretion. Since P is a non-renewable resource, a shortage is imminent due to widespread over-usage in the farming and animal husbandry industries. As a consequence, P efficiency should be improved in pig production. We sought to characterize the transcriptional response in re−/absorbing and excreting tissues in pigs to diets varying in calcium: phosphorus ratios. Weaned piglets were assigned to one of three groups fed diets varying in digestible P content for a period of five weeks. Gene expression profiles were analyzed in jejunum, colon, and kidney. Transcriptome analysis revealed that reduced dietary P intake affects gene expression in jejunum and kidney, but not in colon. The regulation of mineral homeostasis was reflected via altered mRNA abundances of CYP24A1, CYP27A1, TRPM6, SPP1, and VDR in jejunum and kidney. Moreover, lowered abundances of transcripts associated with the classical complement system pathway were observed in the jejunum. In kidney, shifted transcripts were involved in phospholipase C, calcium signaling, and NFAT signaling, which may have immunomodulatory implications. Our results revealed local transcriptional consequences of variable P intake in intestinal and renal tissues. The adaptive responses are the result of organismal efforts to maintain systemic mineral homeostasis while modulating immune features at local tissue sites. Therefore, the deviation from the currently recommended dietary P supply must be carefully considered, as the endogenous mechanisms that respond to low P diets may impact important adaptive immune responses. The online version of this article (10.1186/s12864-018-4584-2) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
5.4
作者：
Freeman TC;Ivens A;Baillie JK;Beraldi D;Barnett MW;Dorward D;Downing A;Fairbairn L;Kapetanovic R;Raza S;Tomoiu A;Alberio R;Wu C;Su AI;Summers KM;Tuggle CK;Archibald AL;Hume DA
通讯作者：
Hume DA
DOI：
10.1016/j.bbrc.2003.09.174
发表时间：
2003-11-28
影响因子：
3.1
作者：
Feske, S;Okamura, H;Rao, A
通讯作者：
Rao, A
影响因子：
--
作者：
CLARK, I
通讯作者：
CLARK, I
半个世纪的全球磷流动、库存、生产、消费、回收和环境影响
DOI：
10.1016/j.gloenvcha.2015.12.005
发表时间：
2016-01-01
影响因子：
8.9
作者：
Chen, Minpeng;Graedel, T. E.
通讯作者：
Graedel, T. E.
DOI：
10.1016/s0301-6226(99)00009-3
发表时间：
1999-04-30
期刊：
LIVESTOCK PRODUCTION SCIENCE
影响因子：
--
作者：
Dourmad, JY;Guingand, N;Sève, B
通讯作者：
Sève, B