Detection of neurochemicals with enhanced sensitivity and selectivity via hybrid multiwall carbon nanotube-ultrananocrystalline diamond microelectrodes.
Detection of neurochemicals with enhanced sensitivity and selectivity via hybrid multiwall carbon nanotube-ultrananocrystalline diamond microelectrodes.
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通过混合多壁碳纳米管-超纳米晶金刚石微电极以增强的灵敏度和选择性检测神经化学物质。
DOI：
10.1016/j.snb.2017.11.054
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Arumugam,PrabhuU
中科院分区：
文献类型：
--
作者：
Tan,Chao;Dutta,Gaurab;Yin,Haocheng;Siddiqui,Shabnam;Arumugam,PrabhuU
异常的神经化学信号通常是大脑疾病的根本原因。电化学微传感器被广泛用于监测具有高时空分辨率的神经化学物质。然而，它们依赖于碳纤维微电极，这往往限制了它们的传感性能。在这项研究中，我们证明了混合多壁碳纳米管（MWCNT）膜修饰的硼掺杂超纳米金刚石（UNCD）微电极（直径250 μm）微传感器的潜力，以改善常见干扰物存在下的多巴胺（DA）的检测。采用电泳沉积（EPD）技术制备了一系列不同膜厚的修饰微电极，并通过扫描电子显微镜、X射线光电子能谱、电化学阻抗谱和银沉积成像对其进行了表征。用循环伏安法，100 nm“薄”膜微电极产生的DA灵敏度值为36 ± 2% μA/μM/cm 2，线性范围为33 nM ~ 1 μM，检测限（LOD）为9.5 ± 1.2% nM。这些微电极的EIS光谱显示三个区域具有不均匀的孔几何形状和不同的阻抗值和电化学活性，这被认为是膜厚度依赖性。使用差分脉冲伏安法，修饰的微电极表现出优异的选择性，表现出三个不同的峰的DA，血清素和过量的抗坏血酸在三元混合物。这些结果提供了两个关键益处：首先，DA灵敏度（>125倍）、选择性（>2000倍）和LOD（>180倍）的显著改善，其次，这些MWCNT可以用简单、可扩展和低成本的EPD工艺选择性地涂覆，用于高度多路复用的微传感器技术。这些进展为化学神经科学的进一步发展提供了相当大的希望。
Abnormal neurochemical signaling is often the underlying cause of brain disorders. Electrochemical microsensors are widely used to monitor neurochemicals with high spatial-temporal resolution. However, they rely on carbon fiber microelectrodes that often limit their sensing performance. In this study, we demonstrate the potential of a hybrid multiwall carbon nanotube (MWCNT) film modified boron-doped ultrananocrystalline diamond (UNCD) microelectrode (250 μm diameter) microsensor for improved detection of dopamine (DA) in the presence of common interferents. A series of modified microelectrodes with varying film thicknesses were microfabricated by electrophoretic deposition (EPD) and characterized by scanning electron microscopy, x-ray photoelectron spectroscopy, electrochemical impedance spectroscopy (EIS) and silver deposition imaging. Using cyclic voltammetry, the 100-nm “thin” film microelectrode produced the most favorable combination of DA sensitivity value of 36 ± 2% μA/μM/cm2with a linear range of 33 nM to 1 μM and a limit of detection (LOD) of 9.5 ± 1.2% nM. The EIS spectra of these microelectrodes revealed three regions with inhomogeneous pore geometry and differing impedance values and electrochemical activity, which was found to be film thickness dependent. Using differential pulse voltammetry, the modified microelectrode showed excellent selectivity by exhibiting three distinct peaks for the DA, serotonin and excess ascorbic acid in a ternary mixture. These results provide two key benefits: first, remarkable improvements in DA sensitivity (>125-fold), selectivity (>2000-fold) and LOD (>180-fold), second, these MWCNTs can be selectively coated with a simple, scalable and low cost EPD process for highly multiplexed microsensor technologies. These advances offer considerable promise for further progress in chemical neurosciences.
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影响因子：
7.4
作者：
Zachek, Matthew K.;Takmakov, Pavel;Moody, Benjamin;Wightman, R. Mark;McCarty, Gregory S.
通讯作者：
McCarty, Gregory S.
综述：传感用碳纳米管混合组件的最新进展
影响因子：
12.6
作者：
Jun Luo;Yong Wu;Shen Lin
通讯作者：
Shen Lin
影响因子：
4
作者：
Arumugam, Prabhu U.;Zeng, Hongjun;Garris, Paul A.
通讯作者：
Garris, Paul A.
影响因子：
7.4
作者：
Pihel, K;Walker, QD;Wightman, RM
通讯作者：
Wightman, RM
DOI：
10.1039/c1an15025a
发表时间：
2011-05-07
期刊：
The Analyst
影响因子：
--
作者：
Koehne JE;Marsh M;Boakye A;Douglas B;Kim IY;Chang SY;Jang DP;Bennet KE;Kimble C;Andrews R;Meyyappan M;Lee KH
通讯作者：
Lee KH