Cytoplasm protein GFAP magnetic beads construction and application as cell separation target for brain tumors.
Cytoplasm protein GFAP magnetic beads construction and application as cell separation target for brain tumors.
复制标题
细胞质蛋白GFAP磁珠的构建及其作为脑肿瘤细胞分离靶点的应用
DOI：
10.1186/s12951-020-00729-9
复制
发表时间：
2020-11-18
影响因子：
10.2
通讯作者：
Ma J
中科院分区：
文献类型：
--
作者：
Zhao Y;Jiang F;Wang Q;Wang B;Han Y;Yang J;Wang J;Wang K;Ao J;Guo X;Liang X;Ma J
背景开发具有诊断和预测功能的高效脑脊液（CSF）检测系统非常重要，其中脑脊液中循环肿瘤细胞（CTC）的检测是一个很好的选择。与过去使用上皮 EpCAM 作为 CTC 分离目标不同，首先选择 GFAP 抗体的细胞质蛋白来构建高灵敏度的免疫磁性脂质体珠 (IML)。在体外和体内测量了该系统捕获脑肿瘤 CTC 的验证和效率。进一步分析患者中CTC数量与其临床特征之间的关联。结果我们的数据显示，可以从32名脑肿瘤儿童的脑脊液和血液样本中成功分离出CTC。脑脊液中CTC的数量明显高于血液中的CTC。脑脊液中 CTC 的水平与肿瘤的类型和位置有关，而不是与肿瘤的分期有关。 CTC数量越高，患者预后不良的可能性就越大。通过桑格测序、q-PCR 和 NGS 方法对 GFAP CTC-DNA 进行基因检测，表明分离的 CTC（GFAP+/EGFR+）是相关肿瘤细胞。例如，脑脊液CTCs中NPR3基因的高表达与肿瘤组织一致。结论结果表明，GFAP-IML CTCs分离系统结合抗肿瘤标志物EGFR免疫荧光检测，可以作为脑肿瘤CTCs鉴定的全新方法。通过腰椎穿刺这种微创手术，该技术可能在脑肿瘤的临床诊断和药物评价中发挥重要作用。
BackgroundIt is very important to develop a highly efficient cerebrospinal fluid (CSF) detection system with diagnosis and prediction function, for which the detection of circulating tumor cells (CTCs) in CSF is a good choice. In contrast to the past use of epithelial EpCAM as CTCs separation target, a cytoplasm protein of GFAP antibody was first selected to construct highly-sensitive immunomagnetic liposome beads (IMLs). The validation and efficiency of this system in capturing CTCs for brain tumors were measured both in vitro and in vivo. The associations between the numbers of CTCs in patients with their clinical characteristics were further analyzed.ResultsOur data show that CTCs can be successfully isolated from CSF and blood samples from 32 children with brain tumors. The numbers of CTCs in CSF were significantly higher than those in blood. The level of CTCs in CSF was related to the type and location of the tumor rather than its stage. The higher the CTCs number is, the more possibly the patient will suffer from poor prognosis. Genetic testing in GFAP CTC-DNA by sanger sequencing, q-PCR and NGS methods indicated that the isolated CTCs (GFAP+/EGFR+) are the related tumor cell. For example, the high expression of NPR3 gene in CSF CTCs was consistent with that of tumor tissue.ConclusionsThe results indicated that GFAP-IML CTCs isolation system, combined with an EGFR immunofluorescence assay of antitumor marker, can serve as a brand-new method for the identification of CTCs for brain tumors. Via lumbar puncture, a minimally invasive procedure, this technique may play a significant role in the clinical diagnosis and drug evaluation of brain tumors.
登录
查看更多内容
影响因子：
3.9
作者：
Au SH;Edd J;Haber DA;Maheswaran S;Stott SL;Toner M
通讯作者：
Toner M
具有脂质双层的表皮生长因子受体肽磁性纳米囊泡的构建用于增强肝癌循环肿瘤细胞的捕获
影响因子：
7.4
作者：
Ding, Jian;Wang, Kai;Liang, Xiao-Fei
通讯作者：
Liang, Xiao-Fei
影响因子：
5.2
作者：
Hanssen, Annkathrin;Riebensahm, Carlotta;Wikman, Harriet
通讯作者：
Wikman, Harriet
影响因子：
8.8
作者：
Krol I;Castro-Giner F;Maurer M;Gkountela S;Szczerba BM;Scherrer R;Coleman N;Carreira S;Bachmann F;Anderson S;Engelhardt M;Lane H;Evans TRJ;Plummer R;Kristeleit R;Lopez J;Aceto N
通讯作者：
Aceto N
影响因子：
28.2
作者：
Haber DA;Velculescu VE
通讯作者：
Velculescu VE