The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection.
The expression profile of Aedes albopictus miRNAs is altered by dengue virus serotype-2 infection.
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白纹伊蚊 miRNA 的表达谱因登革热病毒血清型 2 感染而改变
DOI：
10.1186/s13578-015-0009-y
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发表时间：
2015
影响因子：
7.5
通讯作者：
Chen XG
中科院分区：
文献类型：
--
作者：
Liu Y;Zhou Y;Wu J;Zheng P;Li Y;Zheng X;Puthiyakunnon S;Tu Z;Chen XG
白纹伊蚊是登革病毒（DENV）的重要传播媒介，在全球热带和温带环境中迅速入侵。一些研究表明，microRNA（miRNAs）调节蚊子对病原体的防御。然而，目前还没有系统分析DENV感染对Ae.白纹伊蚊我们进行了这项研究，以研究Ae的miRNA表达。使用Illumina RNA测序检测白纹伊蚊感染DENV-2的情况。在DENV-2感染和未感染的成年雌性Ae中共鉴定了103种已知的和5种新的候选miRNAs。白纹伊蚊比较分析表明，感染后52个miRNAs表达显著下调，18个表达显著上调。此外，RT-qPCR验证了这些差异表达的miRNA中的11个的表达模式。对这些调控的miRNAs的靶点预测和功能分析表明，miR-34- 5 p和miR-87可能参与了抗病原体和免疫应答。这是首次系统研究DENV感染对Ae中miRNA表达的影响。白纹伊蚊miRNA表达的复杂变化表明miRNA通过调节免疫相关基因在抗病毒反应中的潜在作用。这项研究提供了有关DENV诱导的miRNAs的信息，并为识别基于载体的抗病毒策略的潜在候选者提供了线索。本文的在线版本（doi：10.1186/s13578-015-0009-y）包含补充材料，可供授权用户使用。
Aedes albopictus is an important vector of Dengue virus (DENV) and it has quickly invaded the tropical and temperate environments worldwide. A few studies have shown that, microRNAs (miRNAs) regulate mosquito defense against pathogens. However, there is no systematic analysis of the impact of DENV infection on miRNA expression in Ae. albopictus. We conducted this study to investigate the miRNA expression of Ae. albopictus upon DENV-2 infection using Illumina RNA sequencing. A total of 103 known and 5 novel candidate miRNAs were identified in DENV-2 infected and non-infected adult female Ae. albopictus. Comparative analysis indicated that 52 miRNAs were significantly down-regulated and 18 were up-regulated significantly after infection. Furthermore, RT-qPCR validated the expression patterns of eleven of these differentially expressed miRNAs. Targets prediction and functional analysis of these regulated miRNAs suggested that miR-34-5p and miR-87 might be involved in the anti-pathogen and immune responses. This is the first systematic study on the impact of DENV infection on miRNA expression in Ae. albopictus. Complex changes in miRNA expression suggest a potential role of miRNAs in antiviral responses by regulating immune-related genes. This investigation provides information concerning DENV-induced miRNAs and offers clues for identifying potential candidates for vector based antiviral strategies. The online version of this article (doi:10.1186/s13578-015-0009-y) contains supplementary material, which is available to authorized users.
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影响因子：
2.6
作者：
Campbell CL;Harrison T;Hess AM;Ebel GD
通讯作者：
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影响因子：
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作者：
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DOI：
10.1073/pnas.1116932108
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Mirbase：MicroRNA基因组学的工具。
影响因子：
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作者：
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通讯作者：
Enright, Anton J.
DOI：
10.1073/pnas.0406983101
发表时间：
2004-12-07
影响因子：
11.1
作者：
Keene, KM;Foy, BD;Olson, KE
通讯作者：
Olson, KE