Intestinal absorption of fluorescently labeled nanoparticles.
Intestinal absorption of fluorescently labeled nanoparticles.
复制标题
DOI：
10.1016/j.nano.2015.02.016
复制
发表时间：
2015-07
期刊：
影响因子：
--
通讯作者：
Desai TA
中科院分区：
文献类型：
--
作者：
Simovic S;Song Y;Nann T;Desai TA
Characterization of intestinal absorption of nanoparticles is critical in the design of noninvasive anticancer, protein-based, and gene nanoparticle-based therapeutics. Here we demonstrate a general approach for the characterization of the intestinal absorption of nanoparticles and for understanding the mechanisms active in their processing within healthy intestinal cells. It is generally accepted that the cellular processing represents a major drawback of current nanoparticle-based therapeutic systems. In particular, endolysosomal trafficking causes degradation of therapeutic molecules such as proteins, lipids, acid-sensitive anticancer drugs, and genes. To date, investigations into nanoparticle processing within intestinal cells have studied mass transport through Caco-2 cells or everted rat intestinal sac models. We developed an approach to visualize directly the mechanisms of nanoparticle processing within intestinal tissue. These results clearly identify a mechanism by which healthy intestinal cells process nanoparticles and point to the possible use of this approach in the design of noninvasive nanoparticle-based therapies. Highlight of a general approach for the characterization of the intestinal absorption of nanoparticles and for understanding the mechanisms active in their processing within healthy intestinal cells: time-dependent visualization and quantification of nanoparticle endocytic uptake through histological cross-sectioning of healthy rat intestinal tissue.
登录
查看更多内容
影响因子：
3.7
作者：
van Hell, Albert J.;Crommelin, Daan J. A.;Hennink, Wim E.;Mastrobattista, Enrico
通讯作者：
Mastrobattista, Enrico
影响因子：
5.4
作者：
Cesur, Hacer;Rubinstein, Israel;Pai, Ashwini;Oenyueksel, Hayat
通讯作者：
Oenyueksel, Hayat
影响因子：
17.1
作者：
Bramini, Mattia;Ye, Dong;Dawson, Kenneth A.
通讯作者：
Dawson, Kenneth A.
影响因子：
64.5
作者：
Lakadamyali, M;Rust, MJ;Zhuang, XW
通讯作者：
Zhuang, XW
影响因子：
4.7
作者：
Li, Ya;Chase, Anna R.;Yin, Hang
通讯作者：
Yin, Hang