No evidence that plasmablasts transdifferentiate into developing neutrophils in severe COVID-19 disease.
No evidence that plasmablasts transdifferentiate into developing neutrophils in severe COVID-19 disease.
复制标题
没有证据表明在严重的COVID-19疾病中，质母细胞转化为发育中的中性粒细胞。
DOI：
10.1002/cti2.1308
复制
发表时间：
2021
影响因子：
5.8
通讯作者：
Phan TG
中科院分区：
文献类型：
--
作者：
Alquicira-Hernandez J;Powell JE;Phan TG
Wilk等人最近的一项单细胞RNA测序研究表明，在患有严重COVID-19疾病的患者中，浆母细胞可以转分化为“发育中的中性粒细胞”。我们为此探索证据。我们下载了作者在研究中使用的原始数据和代码，以复制他们的发现，并探索回归变量可能导致作者过度拟合数据的可能性。当关键特征未回归时，浆母细胞和发育中的中性粒细胞之间的谱系关系破裂，并且在分析期间数据未过度拟合。浆母细胞不转分化为发育中的中性粒细胞。单细胞RNA测序是生物发现和假设生成的强大技术。然而，在生物信息学分析和解释通过正交技术交叉验证的数据和结果时应谨慎。单细胞RNA测序可以产生意想不到的假设。然而，这些应该通过正交技术进行严格的测试和交叉验证。在这种情况下，没有证据表明浆母细胞在严重的COVID-19疾病中分化为中性粒细胞。
A recent single‐cell RNA sequencing study by Wilk et al. suggested that plasmablasts can transdifferentiate into ‘developing neutrophils’ in patients with severe COVID‐19 disease. We explore the evidence for this. We downloaded the original data and code used by the authors in their study to replicate their findings and explore the possibility that regressing out variables may have led the authors to overfit their data. The lineage relationship between plasmablasts and developing neutrophils breaks down when key features are not regressed out, and the data are not overfitted during the analysis. Plasmablasts do not transdifferentiate into developing neutrophils. The single‐cell RNA sequencing is a powerful technique for biological discovery and hypothesis generation. However, caution should be exercised in the bioinformatic analysis and interpretation of the data and findings cross‐validated by orthogonal techniques. Single cell RNA sequencing can generate unexpected hypotheses. However, these should be rigorously tested and cross‐validated by orthogonal techniques. In this case, there is no evidence that plasmablasts differentiate into neutrophils in severe COVID‐19 disease.
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