Human keratinocytes contain mRNA indistinguishable from monocyte interleukin 1 alpha and beta mRNA. Keratinocyte epidermal cell-derived thymocyte-activating factor is identical to interleukin 1.
Human keratinocytes contain mRNA indistinguishable from monocyte interleukin 1 alpha and beta mRNA. Keratinocyte epidermal cell-derived thymocyte-activating factor is identical to interleukin 1.
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DOI：
10.1084/jem.164.6.2095
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发表时间：
1986-12-01
期刊：
影响因子：
--
通讯作者：
Gubler U
中科院分区：
文献类型：
--
作者：
Kupper TS;Ballard DW;Chua AO;McGuire JS;Flood PM;Horowitz MC;Langdon R;Lightfoot L;Gubler U
角质形成细胞产生IL-1样因子，称为表皮细胞源性胸腺细胞活化因子（ETAF）。在这项研究中，我们通过以下标准证明了ETAF和IL-1是相同的：通过S1核酸酶保护试验确定，正常和恶性人角质形成细胞含有与单核细胞IL-1 α和IL-1 β mRNA相同的mRNA；在这些细胞调节的培养基中，IL-1活性可以被人类IL-1特异性抗体中和。IL-1 α和IL-1 β mrna可以在没有可识别刺激的情况下在培养的人角质形成细胞中被识别；这种基础水平的mRNA可以通过某些特定的刺激进一步诱导积累。培养的正常人角化细胞（HFKs）中IL-1 α的含量是IL-1 β mRNA的2-4倍；相比之下，人外周血单核细胞中IL-1 β的含量是IL-1 α mRNA的10-20倍。这些HFK释放的IL-1活性可以被一种同时中和α和β IL-1的抗体所中和，但不能被一种只中和IL-1的抗体所中和。虽然人单核细胞在适当刺激后产生大量过量的IL-1 β，但这些数据表明，IL-1 α是人角质形成细胞产生的IL-1的主要（可能是主要）形式。
Keratinocytes produce an IL-1 like factor termed epidermal cell-derived thymocyte-activating factor (ETAF). In this study, we show that ETAF and IL-1 are identical by the following criteria: Both normal and malignant human keratinocytes contain mRNAs identical to monocytic IL-1 alpha and IL-1 beta mRNA, as determined by an S1 nuclease protection assay; and IL-1 activity in medium conditioned by these cells can be neutralized by antibodies specific for human IL-1. The IL-1 alpha and IL-1 beta mRNAs can be identified in cultured human keratinocytes in the absence of identifiable stimulation; this basal level of mRNA can be further induced to accumulate with certain defined stimuli. Cultured normal human keratinocytes (HFKs) contain 2-4 times more IL-1 alpha than IL-1 beta mRNA; in contrast, human peripheral blood monocytes contain 10-20 times more IL-1 beta than IL-1 alpha mRNA. The IL-1 activity released by these HFK can be neutralized by an antibody that neutralizes both alpha and beta IL-1, but not by an antibody that neutralizes only IL-1 beta. While human monocytes produce a large excess of IL-1 beta after appropriate stimulation, these data suggest that IL-1 alpha is a major (and may be the predominant) form of IL-1 produced by human keratinocytes.
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影响因子：
64.8
作者：
LOMEDICO, PT;GUBLER, U;MIZEL, SB
通讯作者：
MIZEL, SB
影响因子：
64.5
作者：
RHEINWALD, JG;GREEN, H
通讯作者：
GREEN, H
影响因子：
2.2
作者：
DUFF, GW;ATKINS, E
通讯作者：
ATKINS, E
影响因子：
15.9
作者：
GAHRING, LC;BUCKLEY, A;DAYNES, RA
通讯作者：
DAYNES, RA
影响因子：
15.9
作者：
DINARELLO, CA;RENFER, L;WOLFF, SM
通讯作者：
WOLFF, SM