Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp.
Signaling events induced by lipopolysaccharide-activated Toll in response to bacterial infection in shrimp.
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DOI：
10.3389/fimmu.2023.1119879
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发表时间：
2023
影响因子：
7.3
通讯作者：
Li, Chaozheng
中科院分区：
文献类型：
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作者：
Wang, Sheng;Li, Haoyang;Li, Qinyao;Yin, Bin;Li, Sedong;He, Jianguo;Li, Chaozheng
Toll样受体（TLR）在脊椎动物和无脊椎动物微生物感染的检测中起着至关重要的作用。哺乳动物TLR直接识别多种结构保守的微生物组分。然而，果蝇等无脊椎动物通过与类精氨酸配体Spätzle结合来间接识别微生物产物，这激活了尚未完全理解的信号级联。在这项研究中，我们调查的信号事件触发Toll响应脂多糖（LPS），革兰氏阴性菌的细胞壁成分，副溶血性弧菌感染的节肢动物虾凡纳滨对虾。我们发现L. LvToll 1、LvToll 2、LvToll 3、LvToll 5和LvToll 9的RNAi减弱了副溶血弧菌诱导的LvDorsal-L磷酸化。所有9种Toll均通过TIR结构域与MyD 88结合，从而将信号传递给肿瘤坏死因子受体相关因子6（TRAF 6）-转化生长因子-β激活激酶1结合蛋白2（TAB 2）-转化生长因子-β激活激酶1（TAK 1）复合物。进一步的研究表明，LvTRAF 6-LvTAB 2-LvTAK 1复合物有助于副溶血性弧菌感染期间Dorsal-L磷酸化和核转位。总体而言，虾Toll 1/2/3/5/9-TRAF 6/TAB 2/TAK 1-Dorsal级联保护宿主免受副溶血性弧菌感染，这提供了对先天免疫系统如何识别和响应无脊椎动物中的细菌感染的更好理解。
Toll-like receptors (TLR) play a crucial role in the detection of microbial infections in vertebrates and invertebrates. Mammalian TLRs directly recognize a variety of structurally conserved microbial components. However, invertebrates such as Drosophila indirectly recognize microbial products by binding to the cytokine-like ligand Spätzle, which activates signaling cascades that are not completely understood. In this study, we investigated the signaling events triggered by Toll in response to lipopolysaccharide (LPS), a cell wall component of gram-negative bacteria, and Vibrio parahaemolyticus infection in the arthropod shrimp Litopenaeus vannamei. We found that five of the nine Tolls from L. vannamei bound to LPS and the RNAi of LvToll1, LvToll2, LvToll3, LvToll5, and LvToll9 weakened LvDorsal-L phosphorylation induced by V. parahaemolyticus. All nine Tolls combined with MyD88 via the TIR domain, thereby conferring signals to the tumor necrosis factor receptor-associated factor 6 (TRAF6)-transforming growth factor-β activated kinase 1 binding protein 2 (TAB2)-transforming growth factor-β activated kinase 1 (TAK1) complex. Further examination revealed that the LvTRAF6-LvTAB2-LvTAK1 complex contributes to Dorsal-L phosphorylation and nuclear translocation during V. parahaemolyticus infection. Overall, shrimp Toll1/2/3/5/9–TRAF6/TAB2/TAK1–Dorsal cascades protect the host from V. parahaemolyticus infection, which provides a better understanding of how the innate immune system recognizes and responds to bacterial infections in invertebrates.
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影响因子：
6.7
作者：
Mitri C;Jacques JC;Thiery I;Riehle MM;Xu J;Bischoff E;Morlais I;Nsango SE;Vernick KD;Bourgouin C
通讯作者：
Bourgouin C
影响因子：
64.5
作者：
Chevrier N;Mertins P;Artyomov MN;Shalek AK;Iannacone M;Ciaccio MF;Gat-Viks I;Tonti E;DeGrace MM;Clauser KR;Garber M;Eisenhaure TM;Yosef N;Robinson J;Sutton A;Andersen MS;Root DE;von Andrian U;Jones RB;Park H;Carr SA;Regev A;Amit I;Hacohen N
通讯作者：
Hacohen N
影响因子：
29.7
作者：
Brubaker SW;Bonham KS;Zanoni I;Kagan JC
通讯作者：
Kagan JC
影响因子：
64.8
作者：
Alexopoulou, L;Holt, AC;Flavell, RA
通讯作者：
Flavell, RA
影响因子：
11
作者：
通讯作者：
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