Land Management Legacy Affects Abundance and Function of the acdS Gene in Wheat Root Associated Pseudomonads.
Land Management Legacy Affects Abundance and Function of the acdS Gene in Wheat Root Associated Pseudomonads.
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DOI：
10.3389/fmicb.2021.611339
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发表时间：
2021
影响因子：
5.2
通讯作者：
Mauchline TH
中科院分区：
文献类型：
--
作者：
Ruscoe HL;Taketani RG;Clark IM;Lund G;Hughes D;Dodd IC;Hirsch PR;Mauchline TH
由于土壤性质的化学、物理和生物改变，土地管理实践可以极大地影响地下植物性状。有益假单胞菌属是具有大量植物生长促进（PGP）品质的农业相关细菌，包括通过细菌酶1-氨基环丙烷-1-羧酸（ACC）脱氨酶（acdS）的作用调节植物产生的乙烯来改变植物生理学的潜力。本研究评估了土地管理遗产对小麦根相关可培养假单胞菌菌株的选择和功能的影响。三个不同的小麦栽培（草地，耕地，和裸露的休闲）之前的土地利用进行了测试和可培养的假单胞菌丰度，pseudogeny（gyrB和acdS基因），功能（ACC脱氨酶活性），并与其他PGP基因的acdS的共选择检查。假单胞菌群落在一定程度上可以根据以前的土地利用进行区分。小麦根际和根室的菌株有一个较高的acdS基因频率比散装土壤，特别是在植物生长在土壤中的裸露休闲处理，这是已知的退化的土壤性质，如低养分的可用性。此外，其他感兴趣的基因，农业编码抗真菌代谢产物，铁载体，和参与氮代谢的基因与acdS基因的存在高度正相关，在长期的耕地处理在这些菌株的基因组。相反，参与抗生素抗性和VI型分泌系统的基因沿着氮循环基因与裸休闲分离的假单胞菌acdS基因高度正相关。这表明，小麦栽培前的三种土地管理方式存在不同的选择压力，可以直接或间接地通过小麦根系的影响来塑造可培养假单胞菌群落的结构和功能。
Land management practices can vastly influence belowground plant traits due to chemical, physical, and biological alteration of soil properties. Beneficial Pseudomonas spp. are agriculturally relevant bacteria with a plethora of plant growth promoting (PGP) qualities, including the potential to alter plant physiology by modulating plant produced ethylene via the action of the bacterial enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase (acdS). This study evaluated the impact of land management legacy on the selection and function of wheat root associated culturable pseudomonad isolates. Three distinct previous land uses prior to wheat culture (grassland, arable, and bare fallow) were tested and culturable pseudomonad abundance, phylogeny (gyrB and acdS genes), function (ACC deaminase activity), and the co-selection of acdS with other PGP genes examined. The pseudomonad community could to some extent be discriminated based on previous land use. The isolates from rhizosphere and root compartments of wheat had a higher acdS gene frequency than the bulk soil, particularly in plants grown in soil from the bare fallow treatment which is known to have degraded soil properties such as low nutrient availability. Additionally, other genes of interest to agriculture encoding anti-fungal metabolites, siderophores, and genes involved in nitrogen metabolism were highly positively associated with the presence of the acdS gene in the long-term arable treatment in the genomes of these isolates. In contrast, genes involved in antibiotic resistance and type VI secretion systems along with nitrogen cycling genes were highly positively correlated with the acdS gene in bare fallow isolated pseudomonad. This highlights that the three land managements prior to wheat culture present different selection pressures that can shape culturable pseudomonad community structure and function either directly or indirectly via the influence of wheat roots.
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影响因子：
5.6
作者：
Hol WH;Bezemer TM;Biere A
通讯作者：
Biere A
DOI：
10.1073/pnas.1301421110
发表时间：
2013-11-05
影响因子：
11.1
作者：
Agren, Jon;Hellstrom, Frida;Ehrlen, Johan
通讯作者：
Ehrlen, Johan
DOI：
10.1016/b978-0-444-63987-5.00010-4
发表时间：
2018-01-01
期刊：
NEW AND FUTURE DEVELOPMENTS IN MICROBIAL BIOTECHNOLOGY AND BIOENGINEERING: CROP IMPROVEMENT THROUGH MICROBIAL BIOTECHNOLOGY
影响因子：
--
作者：
David, Baliah V.;Chandrasehar, Govindan;Selvam, Pamila N.
通讯作者：
Selvam, Pamila N.
影响因子：
5.2
作者：
Kavamura, Vanessa Nessner;Robinson, Rebekah J.;Mauchline, Tim H.
通讯作者：
Mauchline, Tim H.
影响因子：
7.4
作者：
Díaz, J;ten Have, A;van Kan, JAL
通讯作者：
van Kan, JAL