Dietary salecan reverts partially the metabolic gene expressions and NMR-based metabolomic profiles from high-fat-diet-induced obese rats.
Dietary salecan reverts partially the metabolic gene expressions and NMR-based metabolomic profiles from high-fat-diet-induced obese rats.
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饮食可以部分恢复高脂肪饮食诱导的肥胖大鼠的代谢基因表达和基于核磁共振的代谢组图谱。
DOI：
10.1016/j.jnutbio.2017.04.015
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发表时间：
2017-09
期刊：
影响因子：
--
通讯作者：
Jianfa Zhang
中科院分区：
文献类型：
--
作者：
Qi Sun;Minghui Li;Xiao Yang;Xi Xu;Junsong Wang;Jianfa Zhang
先前的研究表明，膳食salecan（一种水溶性β-葡聚糖）通过干扰小鼠胆汁酸促进的乳化作用，有效降低高脂饮食诱导的肥胖。然而，salecan对参与脂质积累的代谢基因和代谢物的影响大多是未知的。在此，我们证实，饮食3%和6% salecan 4周显著降低高脂饮食大鼠肝脏和脂肪组织中的脂肪积累，显示SREBP 1-C、FAS、SCD 1和ACC 1的mRNA水平降低，这与nde novolipogenesis有关，并且与甘油三酯合成相关的GPAT 1、DGAT 1和DGAT 2的水平降低。膳食salecan还增加了PPAR α和CYP7A1的mRNA水平，这分别与脂肪酸氧化和胆固醇分解有关。在1H核磁共振代谢组学分析中，对照组之间的血清和肝脏代谢物谱存在差异，salecan组的代谢谱向低脂饮食组偏移。代谢物分析表明，salecan显着增加肝脏谷胱甘肽和甜菜碱水平，这是与细胞活性氧的调节。这些数据表明，膳食salecan不仅干扰脂肪的消化和吸收，而且影响脂肪的积累和代谢，在饮食诱导的肥胖。
Previous studies suggest that dietary salecan (a water-soluble β-glucan) effectively reduces high-fat-diet-induced adiposity through disturbing bile-acid-promoted emulsification in mice. However, the effects of salecan on metabolic genes and metabolites involved in lipid accumulation are mostly unknown. Here, we confirmed that dietary 3% and 6% salecan for 4 weeks markedly decreased fat accumulation in liver and adipose tissue in high-fat-diet rats, displaying a decrease in mRNA levels of SREBP1-C, FAS, SCD1 and ACC1 involved inde novolipogenesis and a reduction of levels of GPAT1, DGAT1 and DGAT2 related to triglyceride synthesis. Dietary salecan also increased the mRNA levels of PPARα and CYP7A1, which are related to fatty acid oxidation and cholesterol decomposition, respectively. In the1H nuclear magnetic resonance metabolomic analysis, both the serum and liver metabolite profiles differed among the control groups, and the metabolic profiles of the salecan groups were shifted toward that of the low-fat-diet group. Metabolites analysis showed that salecan significantly increased hepatic glutathione and betaine levels which are related to regulation of cellular reactive oxygen species. These data demonstrate that dietary salecan not only disturbed fat digestion and absorption but also influenced lipid accumulation and metabolism in diet-induced obesity.
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DOI：
10.3945/ajcn.114.086108
发表时间：
2014-12
期刊：
The American journal of clinical nutrition
影响因子：
--
作者：
Whitehead A;Beck EJ;Tosh S;Wolever TM
通讯作者：
Wolever TM
影响因子：
9.8
作者：
Kaczmarczyk, Melissa M.;Miller, Michael J.;Freund, Gregory G.
通讯作者：
Freund, Gregory G.
影响因子：
1.2
作者：
A. Jiménez-Cruz;V. Manuel Loustaunau-López;M. Bacardí-Gascón
通讯作者：
A. Jiménez-Cruz;V. Manuel Loustaunau-López;M. Bacardí-Gascón
影响因子：
5.2
作者：
Choi, Joo Sun;Kim, Hyunsook;Song, Jihyun
通讯作者：
Song, Jihyun
影响因子：
2.4
作者：
H. S. Lim;P. White;K. Frey
通讯作者：
H. S. Lim;P. White;K. Frey