A novel high-throughput imaging system for automated analyses of avoidance behavior in zebrafish larvae.
A novel high-throughput imaging system for automated analyses of avoidance behavior in zebrafish larvae.
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DOI：
10.1016/j.bbr.2011.04.033
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发表时间：
2011-09-30
影响因子：
2.7
通讯作者：
Creton, Robbert
中科院分区：
文献类型：
--
作者：
Pelkowski, Sean D.;Kapoor, Mrinal;Richendrfer, Holly A.;Wang, Xingyue;Colwill, Ruth M.;Creton, Robbert
关键词：
早期大脑发育可能受到许多遗传和环境因素的影响，对大脑功能和行为产生长期影响。最近的高通量筛选创新促进了这些因素的识别。然而，对整个生物体进行大规模筛选仍然具有挑战性，特别是在研究脊椎动物模型系统中大脑功能或行为的变化时。在这项研究中，我们提出了一种新型成像系统，用于对斑马鱼幼虫的行为进行高通量分析。三摄像头系统可以同时对十二个多孔板进行成像，并且其独特之处在于能够在多孔板的孔中提供局部视觉刺激。获取的图像被转换成一系列坐标，表征幼虫的位置和方向。通过测量 7 天大的斑马鱼幼虫的回避行为来测试所开发的成像技术。该系统有效地量化了幼虫的回避行为，并显示出对蓝色或红色“弹跳球”刺激的边缘偏好增加。当幼虫与静止球平衡时，也会避免弹跳球刺激，但不会避免与静止球平衡的闪烁球。这些结果表明，7 天大的幼虫对运动有特异性反应，而不是颜色、大小或光强度的局部变化。用于测量回避行为的成像系统和测定可用于筛选导致发育性脑部疾病的遗传和环境因素以及可以预防或治疗这些疾病的新药物。
Early brain development can be influenced by numerous genetic and environmental factors, with long-lasting effects on brain function and behavior. The identification of these factors is facilitated by recent innovations in high-throughput screening. However, large-scale screening in whole organisms remains challenging, in particular when studying changes in brain function or behavior in vertebrate model systems. In this study, we present a novel imaging system for high-throughput analyses of behavior in zebrafish larvae. The three-camera system can image twelve multiwell plates simultaneously and is unique in its ability to provide local visual stimuli in the wells of a multiwell plate. The acquired images are converted into a series of coordinates, which characterize the location and orientation of the larvae. The developed imaging techniques were tested by measuring avoidance behaviors in seven-day-old zebrafish larvae. The system effectively quantified larval avoidance and revealed an increased edge preference in response to a blue or red ‘bouncing ball’ stimulus. Larvae also avoid a bouncing ball stimulus when it is counter-balanced with a stationary ball, but do not avoid blinking balls counter-balanced with a stationary ball. These results indicate that the seven-day-old larvae respond specifically to movement, rather than color, size, or local changes in light intensity. The imaging system and assays for measuring avoidance behavior may be used to screen for genetic and environmental factors that cause developmental brain disorders and for novel drugs that could prevent or treat these disorders.
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影响因子：
1.3
作者：
Colwill, Ruth M.;Creton, Robbert
通讯作者：
Creton, Robbert
影响因子：
3.7
作者：
Blackiston, Douglas;Shomrat, Tal;Levin, Michael
通讯作者：
Levin, Michael
影响因子：
25
作者：
McLean, David L.;Masino, Mark A.;Koh, Ingrid Y. Y.;Lindquist, W. Brent;Fetcho, Joseph R.
通讯作者：
Fetcho, Joseph R.
影响因子：
14.8
作者：
Kokel, David;Bryan, Jennifer;Laggner, Christian;White, Rick;Cheung, Chung Yan J.;Mateus, Rita;Healey, David;Kim, Sonia;Werdich, Andreas A.;Haggarty, Stephen J.;MacRae, Calum A.;Shoichet, Brian;Peterson, Randall T.
通讯作者：
Peterson, Randall T.
DOI：
10.1126/science.1183090
发表时间：
2010-01-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rihel J;Prober DA;Arvanites A;Lam K;Zimmerman S;Jang S;Haggarty SJ;Kokel D;Rubin LL;Peterson RT;Schier AF
通讯作者：
Schier AF