Locomotor behaviors in zebrafish (Danio rerio) larvae.
Locomotor behaviors in zebrafish (Danio rerio) larvae.
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DOI：
10.1016/j.beproc.2010.12.003
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发表时间：
2011-02
影响因子：
1.3
通讯作者：
Creton, Robbert
中科院分区：
文献类型：
--
作者：
Colwill, Ruth M.;Creton, Robbert
以斑马鱼（Danio rerio）受精后4、5、6和7天的仔鱼为材料，进行了两个实验，研究了斑马鱼仔鱼的运动行为。每天在12孔板的各个威尔斯孔中观察幼虫1小时。在实验1中，从受精后第4天开始连续4天观察相同的幼虫;在实验2中，在4、5、6和7 dpf观察来自相同卵收集的不同组幼虫。每6秒收集的自动图像进行分析，以获得有关幼虫位置，方向和一般活动的信息。在这两个实验中，4 dpf幼虫休息显着更多，更频繁地使用更小面积的井，一般不太活跃，比老幼虫。所有幼虫都表现出偏好背对孔的中心和孔的边缘。然而，长时间暴露在边缘区域会影响整体活动、方向和偏好。这些结果的影响，了解发展的幼虫行为和程序的设计，以衡量经验的影响，在斑马鱼幼虫进行了讨论。
Locomotor behaviors were examined in two experiments using zebrafish (Danio rerio) larvae at 4, 5, 6 and 7 days post fertilization (dpf). Larvae were observed in individual wells of a 12-well plate for one hour a day. In Experiment 1, the same larvae were observed for four consecutive days beginning on post-fertilization day 4; in Experiment 2, different groups of larvae from the same egg collection were observed at 4, 5, 6 and 7 dpf. Automated images collected every 6 seconds were analyzed for information about larval location, orientation and general activity. In both experiments, 4 dpf larvae rested significantly more, used a smaller area of the well more frequently, and were generally less active than older larvae. All larvae exhibited a preference for facing away from the center of the well and for the edge of the well. However, prolonged exposure to the well influenced overall activity, orientation, and preference for the edge region. The implications of these results for understanding the development of larval behavior and for the design of procedures to measure the effects of experience in zebrafish larvae are discussed.
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影响因子：
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