Bacteria responsive antibacterial surfaces for indwelling device infections.
Bacteria responsive antibacterial surfaces for indwelling device infections.
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DOI：
10.1016/j.jconrel.2014.11.025
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发表时间：
2015-01-28
期刊：
影响因子：
--
通讯作者：
Liang JF
中科院分区：
文献类型：
--
作者：
Traba C;Liang JF
由于生物膜对抗生素治疗的耐受性，留置装置感染现在代表危及生命的情况。目前通过持续释放抗生素的抗生素浸渍方法存在一些未解决的问题，包括寿命短，抗菌谱窄，对抗性突变体无效，以及加速抗生素抗性过程的潜力。在这项研究中，细菌响应性抗生物膜表面开发使用生物活性肽与抗生素耐药菌和生物膜的活性证明。所得表面在生理条件下和在高浓度的盐（0.5 M NaCl）和生物大分子（1.0% DNA和2.0%藻酸盐）的存在下是稳定的，从而显示出良好的生物相容性，各种组织细胞。然而，裂解肽固定化表面可以感知细菌的粘附，并有效地和特异性地杀死附着的细菌，因此生物膜不能在裂解肽固定化表面上形成。细菌响应导管在长达一周的时间内保持无生物膜。因此，本研究中开发的细菌响应抗菌表面代表了留置器械感染的新机会。
Indwelling device infections now represents life-threatening circumstances as a result of the biofilms’ tolerance to antibiotic treatments. Current antibiotic impregnation approaches through sustained antibiotic release have some unsolved problems which include short life-span, narrowed antibacterial spectrum, ineffectiveness towards resistant mutants, and the potential to hasten the antibiotic resistance process. In this study, bacteria responsive anti-biofilm surfaces were developed using bioactive peptides with proved activity to antibiotic resistant bacteria and biofilms. Resulting surfaces were stable under physiological conditions and in the presence of high concentrations of salts (0.5 M NaCl) and biomacromolcules (1.0% DNA and 2.0% alginate), and thus showed good biocompatibility to various tissue cells. However, lytic peptide immobilized surfaces could sense bacteria adhesion and kill attached bacteria effectively and specifically, so biofilms were unable to develop on the lytic peptide immobilized surfaces. Bacteria responsive catheters remained biofilm free for up to a week. Therefore, the bacteria responsive antibacterial surfaces developed in this study represent new opportunities for indwelling device infections.
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DOI：
10.1016/j.juro.2010.07.036
发表时间：
2010-12
期刊：
The Journal of urology
影响因子：
--
作者：
Desai DG;Liao KS;Cevallos ME;Trautner BW
通讯作者：
Trautner BW
影响因子：
2.7
作者：
Traba C;Liang JF
通讯作者：
Liang JF
影响因子：
2.9
作者：
Janek, K;Rothemund, S;Krause, E
通讯作者：
Krause, E
影响因子：
2.8
作者：
Kharidia, Riddhi;Tu, Zhigang;Liang, Jun F.
通讯作者：
Liang, Jun F.
影响因子：
1.7
作者：
Van Regenmortel, MHV
通讯作者：
Van Regenmortel, MHV