The pulmonary mycobiome-A study of subjects with and without chronic obstructive pulmonary disease.
The pulmonary mycobiome-A study of subjects with and without chronic obstructive pulmonary disease.
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DOI：
10.1371/journal.pone.0248967
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Nielsen R
中科院分区：
文献类型：
--
作者：
Martinsen EMH;Eagan TML;Leiten EO;Haaland I;Husebø GR;Knudsen KS;Drengenes C;Sanseverino W;Paytuví-Gallart A;Nielsen R
肺部微生物组（真菌生物组）的真菌部分尚未得到充分研究。我们在一项大规模单中心支气管镜检查研究（MicroCOPD）中报告了COPD参与者与对照组的口腔和肺部真菌群落组成。从93名COPD参与者和100名对照者中收集口腔冲洗液和支气管肺泡灌洗液（BAL）。提取真菌DNA，然后对真菌核糖体RNA基因簇的内部转录间隔区1（ITS 1）区域进行测序。分类条形图，我们比较了分类组成，香农指数，β多样性研究组之间，并通过使用吸入类固醇。对照组和COPD受试者的口腔和肺部真菌组以念珠菌为主，两个研究组的口腔样本中念珠菌多于BAL样本。马拉色菌和Sarocladium也经常发现在肺部样本。在不同丰度/分布方面，研究组之间没有发现一致的差异。α和β多样性在肺部样本的研究组之间没有差异，但β多样性随样本类型而变化。真菌群落似乎不受吸入类固醇的影响。口腔和肺部样本的分类组成和多样性不同，可能表明存在肺部真菌群落。
The fungal part of the pulmonary microbiome (mycobiome) is understudied. We report the composition of the oral and pulmonary mycobiome in participants with COPD compared to controls in a large-scale single-centre bronchoscopy study (MicroCOPD). Oral wash and bronchoalveolar lavage (BAL) was collected from 93 participants with COPD and 100 controls. Fungal DNA was extracted before sequencing of the internal transcribed spacer 1 (ITS1) region of the fungal ribosomal RNA gene cluster. Taxonomic barplots were generated, and we compared taxonomic composition, Shannon index, and beta diversity between study groups, and by use of inhaled steroids. The oral and pulmonary mycobiomes from controls and participants with COPD were dominated by Candida, and there were more Candida in oral samples compared to BAL for both study groups. Malassezia and Sarocladium were also frequently found in pulmonary samples. No consistent differences were found between study groups in terms of differential abundance/distribution. Alpha and beta diversity did not differ between study groups in pulmonary samples, but beta diversity varied with sample type. The mycobiomes did not seem to be affected by use of inhaled steroids. Oral and pulmonary samples differed in taxonomic composition and diversity, possibly indicating the existence of a pulmonary mycobiome.
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影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
5.2
作者：
Hoggard M;Vesty A;Wong G;Montgomery JM;Fourie C;Douglas RG;Biswas K;Taylor MW
通讯作者：
Taylor MW
影响因子：
5.4
作者：
Hawksworth DL;Crous PW;Redhead SA;Reynolds DR;Samson RA;Seifert KA;Taylor JW;Wingfield MJ;Abaci O;Aime C;Asan A;Bai FY;de Beer ZW;Begerow D;Berikten D;Boekhout T;Buchanan PK;Burgess T;Buzina W;Cai L;Cannon PF;Crane JL;Damm U;Daniel HM;van Diepeningen AD;Druzhinina I;Dyer PS;Eberhardt U;Fell JW;Frisvad JC;Geiser DM;Geml J;Glienke C;Gräfenhan T;Groenewald JZ;Groenewald M;de Gruyter J;Guého-Kellermann E;Guo LD;Hibbett DS;Hong SB;de Hoog GS;Houbraken J;Huhndorf SM;Hyde KD;Ismail A;Johnston PR;Kadaifciler DG;Kirk PM;Kõljalg U;Kurtzman CP;Lagneau PE;Lévesque CA;Liu X;Lombard L;Meyer W;Miller A;Minter DW;Najafzadeh MJ;Norvell L;Ozerskaya SM;Oziç R;Pennycook SR;Peterson SW;Pettersson OV;Quaedvlieg W;Robert VA;Ruibal C;Schnürer J;Schroers HJ;Shivas R;Slippers B;Spierenburg H;Takashima M;Taşkın E;Thines M;Thrane U;Uztan AH;van Raak M;Varga J;Vasco A;Verkley G;Videira SI;de Vries RP;Weir BS;Yilmaz N;Yurkov A;Zhang N
通讯作者：
Zhang N
影响因子：
15.5
作者：
Davis, Nicole M;Proctor, Diana M;Callahan, Benjamin J
通讯作者：
Callahan, Benjamin J
影响因子：
--
作者：
Mandal S;Van Treuren W;White RA;Eggesbø M;Knight R;Peddada SD
通讯作者：
Peddada SD