Random glycopeptide bead libraries for seromic biomarker discovery.
Random glycopeptide bead libraries for seromic biomarker discovery.
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DOI：
10.1021/pr1008477
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发表时间：
2010-12-03
影响因子：
4.4
通讯作者：
Blixt, Ola
中科院分区：
文献类型：
--
作者：
Kracun, Stjepan K.;Clo, Emiliano;Clausen, Henrik;Levery, Steven B.;Jensen, Knud J.;Blixt, Ola
关键词：
Identification of disease specific biomarkers is important to address early diagnosis and management of disease. Aberrant post-translational modifications (PTM) of proteins such as O-glycosylations (O-PTMs) are emerging as triggers of autoantibodies that can serve as sensitive biomarkers. Here we have developed a random glycopeptide bead library screening platform for detection of autoantibodies and other binding proteins. Libraries were build on biocompatible PEGA beads including a safety-catch C-terminal amide linker (SCAL) that allowed mild cleavage conditions (I2/NaBH4 and TFA) for release of glycopeptides and sequence determination by ESI-MSn. As proof-of principle, tumor specific glycopeptide reporter epitopes were built-in into the libraries and were detected by tumor specific monoclonal antibodies and autoantibodies from cancer patients. Sequenced and identified glycopeptides were re-synthesized at preparative scale by automated parallel peptide synthesis and printed on microarrays for validation and broader analysis with larger sets of sera. We further showed that chemical synthesis of the monosaccharide O-glycopeptide library (Tn-glycoform) could be diversified to other tumor glycoforms by on-bead enzymatic glycosylation reactions with recombinant glycosyltransferases. Hence, we have developed a high-throughput flexible platform for rapid biomarker discovery O-glycopeptides and the method has applicability in other types of assays like lectin/antibody/enzyme specificity studies as well as investigation of other PTMs.
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DOI：
10.1002/psc.310010106
发表时间：
1995-01-01
期刊：
Journal of peptide science : an official publication of the European Peptide Society
影响因子：
--
作者：
Auzanneau, F I;Meldal, M;Bock, K
通讯作者：
Bock, K
影响因子：
--
作者：
Price, MR;Rye, PD;Hilgers, J
通讯作者：
Hilgers, J
影响因子：
11.5
作者：
Sabbatini, Paul J.;Ragupathi, Govind;Livingston, Philip O.
通讯作者：
Livingston, Philip O.
DOI：
10.1007/s00262-009-0733-4
发表时间：
2009-10
期刊：
Cancer immunology, immunotherapy : CII
影响因子：
--
作者：
Reuschenbach M;von Knebel Doeberitz M;Wentzensen N
通讯作者：
Wentzensen N
影响因子：
7
作者：
Olsen, JV;de Godoy, LMF;Mann, M
通讯作者：
Mann, M