In vivo genetic manipulation of the rat trophoblast cell lineage using lentiviral vector delivery.
In vivo genetic manipulation of the rat trophoblast cell lineage using lentiviral vector delivery.
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DOI：
10.1002/dvg.20518
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发表时间：
2009-07
期刊：
影响因子：
1.5
通讯作者：
Soares, Michael J.
中科院分区：
文献类型：
--
作者：
Lee, Dong-Soo;Rumi, M. A. Karim;Konno, Toshihiro;Soares, Michael J.
在本报告中，我们采用慢病毒基因传递技术对大鼠滋养细胞谱系进行遗传修饰。用慢病毒颗粒孵育囊胚，并将囊胚转移到假妊娠雌性大鼠的子宫内，在妊娠的不同时间收获囊胚，然后进行分析。本研究评估了两种测试系统：1)在组成启动子控制下，将增强型绿色荧光蛋白（EGFP）基因递送至大鼠囊胚；2)将EGFP短发夹RNA （shRNA）传递给组成型表达EGFP的大鼠囊胚。慢病毒包装的基因结构被有效地和特异性地传递到所有滋养细胞系。此外，慢病毒介导的shrna转移是一种有效的策略来修饰滋养细胞谱系中的基因表达。该技术将允许在大鼠滋养细胞谱系中对“功能获得”和“功能丧失”操作进行体内评估。
In this report, we have adapted a lentiviral gene delivery technique for genetic modification of the rat trophoblast cell lineage. Blastocysts were incubated with lentiviral particles and transferred into the uteri of pseudopregnant female rats, harvested at various times during gestation, and then analyzed. Two test systems were evaluated: 1) delivery of an enhanced green fluorescent protein (EGFP) gene under the control of constitutive promoters to rat blastocysts; 2) delivery of EGFP short hairpin RNA (shRNA) to rat blastocysts constitutively expressing EGFP. Lentiviral packaged gene constructs were efficiently and specifically delivered to all trophoblast cell lineages. Additionally, lentiviral mediated transfer of shRNAs was an effective strategy for modifying gene expression in trophoblast cell lineages. This technique will permit the in vivo evaluation of ‘gain-of-function’ and ‘loss-of-function’ manipulations in the rat trophoblast cell lineage.
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影响因子：
3.5
作者：
Ikawa, M;Yamada, S;Okabe, M
通讯作者：
Okabe, M
影响因子：
3.5
作者：
Hasuwa, H;Kaseda, K;Okabe, M
通讯作者：
Okabe, M
DOI：
10.1073/pnas.232688199
发表时间：
2003-01-07
影响因子：
11.1
作者：
Qin, XF;An, DS;Baltimore, D
通讯作者：
Baltimore, D
影响因子：
2.7
作者：
Ain, R;Canham, LN;Soares, MJ
通讯作者：
Soares, MJ
影响因子：
5.4
作者：
Dull, T;Zufferey, R;Naldini, L
通讯作者：
Naldini, L