The Fluid Mechanics of Genome Mapping.
The Fluid Mechanics of Genome Mapping.
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DOI：
10.1002/aic.14002
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发表时间：
2013-02-01
期刊：
影响因子：
3.7
通讯作者：
Dorfman, Kevin D.
中科院分区：
文献类型：
--
作者：
Dorfman, Kevin D.
随着化学工程研究变得越来越跨学科，重要的是要确定化学工程师可以做出独特贡献的领域。这一挑战对于起源于生物学的问题尤其明显。这种观点使得化学工程师有一些独特的贡献，通过学科的核心力量在流体力学基因组绘图领域的情况。乍一看，流体流动的动力学与基因组技术如此密切相关似乎令人惊讶，而基因组技术通常与生物化学有关。然而，化学工程在这一领域的研究是该学科对低雷诺数流体力学[1]和复杂流体流变学[2]长期贡献的自然延伸。为了发展这一案例，该观点首先解释了基因组作图及其与DNA测序的联系。由于这种生物学应用可能是不熟悉的流体力学社区，这个问题是重铸作为一个更熟悉的类比与数值方法。在概述了用于创建基因组图谱的三种新方法之后，该观点强调了其中两种方法的化学工程工作的一些最佳例子：DNA在延伸流中和DNA在限制中。这些例子很难代表对文献的全面回顾，对这样的回顾感兴趣的读者应该查阅最近的一篇论文[3]。本观点的结尾讨论了一些非常适合化学工程思维的突出问题。
As chemical engineering research becomes increasingly interdisciplinary, it is important to identify areas where chemical engineers can make a unique contribution. This challenge is particularly manifest for problems that originate in biology. This Perspective makes the case that chemical engineers have something unique to contribute to the area of genome mapping through the discipline’s core strength in fluid mechanics. At first glance, it may seem surprising that the dynamics of fluid flow are so closely related to genomic technologies, which are normally associated with biochemistry. However, chemical engineering research into this area is a natural extension of the discipline’s long standing contributions to low Reynolds number hydrodynamics [1] and the rheology of complex fluids [2].To develop this case, the Perspective begins with an explanation of genome mapping and its connection to DNA sequencing. As this biological application may be unfamiliar to the fluid mechanics community, the problem is recast as a more familiar analogy with numerical methods. After outlining three of the newer approaches used to create genomic maps, the Perspective highlights some of the best examples of chemical engineering work on two of these methods: DNA in extensional flow and DNA in confinement. These examples hardly represent a thorough review of the literature, and readers interested in such a review should consult a recent paper [3]. The end of this Perspective discusses some outstanding questions that are ideally suited to a chemical engineering mindset.
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影响因子：
4.4
作者：
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通讯作者：
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影响因子：
44.1
作者：
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