The ecogenomics of dsDNA bacteriophages in feces of stabled and feral horses.
The ecogenomics of dsDNA bacteriophages in feces of stabled and feral horses.
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DOI：
10.1016/j.csbj.2020.10.036
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发表时间：
2020
影响因子：
6
通讯作者：
Letarov AV
中科院分区：
文献类型：
--
作者：
Babenko VV;Millard A;Kulikov EE;Spasskaya NN;Letarova MA;Konanov DN;Belalov IS;Letarov AV
哺乳动物下消化道的病毒组被证明是噬菌体占主导地位，然而，只有对人类的噬菌体群落的组成和互变性进行了深入研究。在这里，我们提出了一个生态基因组学调查的dsDNA噬菌体的多样性，在马（马caballus）的粪便，比较两组马厩的马，和一个更进一步的一组野马，被隔离在一个岛上。我们的研究结果表明，马粪便中的dsDNA病毒组具有更高的丰富度比在人类病毒组，更均匀的基因型分布。没有发现过度代表的噬菌体基因型，如在人类中发现的CrAsage相关病毒。此外，许多噬菌体属级集群被发现存在于所有三个地理隔离的人群。马肠道噬菌体的多样性严重采样不足，因此只有一小部分噬菌体重叠群可以与噬菌体基因组连接。我们的研究表明，马和人类肠道生态系统中的噬菌体生态参数存在显着差异，导致它们以不同的方式塑造相应的病毒组。因此，不同动物肠道病毒组的多样性和结构需要进行实验研究。
The viromes of the mammalian lower gut were shown to be heavily dominated by bacteriophages; however, only for humans were the composition and intervariability of the bacteriophage communities studied in depth. Here we present an ecogenomics survey of dsDNA bacteriophage diversity in the feces of horses (Equus caballus), comparing two groups of stabled horses, and a further group of feral horses that were isolated on an island. Our results indicate that the dsDNA viromes of the horse feces feature higher richness than in human viromes, with more even distribution of genotypes. No over-represented phage genotypes, such as CrAssphage-related viruses found in humans, were identified. Additionally, many bacteriophage genus-level clusters were found to be present in all three geographically isolated populations. The diversity of the horse intestinal bacteriophages is severely undersampled, and so consequently only a minor fraction of the phage contigs could be linked with the bacteriophage genomes. Our study indicates that bacteriophage ecological parameters in the intestinal ecosystems in horses and humans differ significantly, leading them to shape their corresponding viromes in different ways. Therefore, the diversity and structure of the intestinal virome in different animal species needs to be experimentally studied.
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