Cyclic stretching of soft substrates induces spreading and growth.
Cyclic stretching of soft substrates induces spreading and growth.
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DOI：
10.1038/ncomms7333
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发表时间：
2015-02-23
影响因子：
16.6
通讯作者：
Sheetz, Michael
中科院分区：
文献类型：
--
作者：
Cui, Yidan;Hameed, Feroz M.;Yang, Bo;Lee, Kyunghee;Pan, Catherine Qiurong;Park, Sungsu;Sheetz, Michael
在体内，软组织经常经历拉伸和放松的循环，这可能会影响细胞的行为，而不会改变基质的刚性。为了确定瞬变力是否可以替代刚性矩阵，我们拉伸了软柱阵。令人惊讶的是，在0.01-10 Hz的频率范围内进行1-5%的循环拉伸会导致伸展和应力纤维的形成(最佳为0.1 Hz)，并在拉伸4 h后持续存在。同样，与刚性基质相比，拉伸提高了软柱子上的细胞生长速度。在与成纤维细胞生长相关的可能因素中，肌钙蛋白相关转录因子-A(mrtf-A)在循环拉伸2 h时移至细胞核，停止时则相反；而yap(是相关蛋白)则移动得更晚。MRTF-A或YAP的基因敲除阻止了依赖拉伸的生长。因此，我们认为，在刺激铺展、应力纤维形成和生长方面，从软基质中反复拉伸可以代替硬基质。生长在坚硬的底物上的细胞通过物理线索受到刺激以扩散，产生肌动蛋白应激纤维并增殖。在这里，崔等人。结果表明，在软柱状基质上循环拉伸细胞与在刚性基质上生长具有相同的效果，并导致YAP和MRTF-A的核转位。
In the body, soft tissues often undergo cycles of stretching and relaxation that may affect cell behaviour without changing matrix rigidity. To determine whether transient forces can substitute for a rigid matrix, we stretched soft pillar arrays. Surprisingly, 1–5% cyclic stretching over a frequency range of 0.01–10 Hz caused spreading and stress fibre formation (optimum 0.1 Hz) that persisted after 4 h of stretching. Similarly, stretching increased cell growth rates on soft pillars comparative to rigid substrates. Of possible factors linked to fibroblast growth, MRTF-A (myocardin-related transcription factor-A) moved to the nucleus in 2 h of cyclic stretching and reversed on cessation; but YAP (Yes-associated protein) moved much later. Knockdown of either MRTF-A or YAP blocked stretch-dependent growth. Thus, we suggest that the repeated pulling from a soft matrix can substitute for a stiff matrix in stimulating spreading, stress fibre formation and growth. Cells grown on a stiff substrate are stimulated through physical cues to spread, create actin stress fibres and proliferate. Here Cui et al. show that cyclic stretching cells on a soft pillar substrate has the same effect as growth on a stiff substrate, and results in nuclear translocation of YAP and MRTF-A.
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影响因子：
21.3
作者：
Prager-Khoutorsky, Masha;Lichtenstein, Alexandra;Bershadsky, Alexander D.
通讯作者：
Bershadsky, Alexander D.
影响因子：
56.9
作者：
Unger, MA;Chou, HP;Quake, SR
通讯作者：
Quake, SR
影响因子：
64.5
作者：
Dong, Jixin;Feldmann, Georg;Pan, Duojia
通讯作者：
Pan, Duojia
DOI：
10.1083/jcb.200204153
发表时间：
2002-11-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Galbraith CG;Yamada KM;Sheetz MP
通讯作者：
Sheetz MP
DOI：
10.1126/science.1191035
发表时间：
2010-08-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gilbert PM;Havenstrite KL;Magnusson KE;Sacco A;Leonardi NA;Kraft P;Nguyen NK;Thrun S;Lutolf MP;Blau HM
通讯作者：
Blau HM