Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae.
Endocytic protein Pal1 regulates appressorium formation and is required for full virulence of Magnaporthe oryzae.
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内吞蛋白 Pal1 调节附着胞形成，是稻瘟病菌完全毒力所必需的
DOI：
10.1111/mpp.13149
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发表时间：
2022-01
影响因子：
4.9
通讯作者：
Xing J
中科院分区：
文献类型：
--
作者：
Chen D;Hu H;He W;Zhang S;Tang M;Xiang S;Liu C;Cai X;Hendy A;Kamran M;Liu H;Zheng L;Huang J;Chen XL;Xing J
内吞作用在植物病原真菌感染过程中起着关键作用，但其调控机制仍不清楚。在这里，我们确定了一个假定的内吞相关基因，PAL 1，这是高度表达的附着胞的Magnaporthe的，并被发现是重要的附着胞的形成和成熟。PAL 1的缺失显著降低了M. lymphocyte的毒力，这是由于在宿主细胞中的附着胞穿透和侵入性生长的缺陷。Pal 1蛋白与内吞作用蛋白Sal 1相互作用并共定位，表明其参与内吞作用。Δ pal 1突变体的附着胞形成显著减少，通过添加外源cAMP和3-异丁基-1-甲基黄嘌呤（IBMX）可以恢复。此外，Δ pal 1中Pmk 1的磷酸化水平也降低，表明Pal 1在cAMP和Pmk 1信号通路的上游发挥作用。因此，在Δ pal 1突变体中，糖原和脂质的利用、附着胞自噬、肌动蛋白环的形成、septin蛋白的定位以及膨压积累都受到影响。综上所述，Pal 1调节cAMP和Pmk 1信号通路，用于附着胞的形成和成熟，以促进M.稻瘟病菌的内吞蛋白Pal 1通过调节cAMP和Pmk 1信号通路促进附着胞的形成和成熟而发挥致病作用。
Endocytosis plays key roles during infection of plant‐pathogenic fungi, but its regulatory mechanisms are still largely unknown. Here, we identified a putative endocytosis‐related gene, PAL1, which was highly expressed in appressorium of Magnaporthe oryzae, and was found to be important for appressorium formation and maturation. Deletion of PAL1 significantly reduced the virulence of M. oryzae due to defects in appressorial penetration and invasive growth in host cells. The Pal1 protein interacted and colocalized with the endocytosis protein Sla1, suggesting it is involved in endocytosis. The Δpal1 mutant was significantly reduced in appressorium formation, which was recovered by adding exogenous cAMP and 3‐isobutyl‐1‐methylxanthine (IBMX). Moreover, the phosphorylation level of Pmk1 in Δpal1 was also reduced, suggesting Pal1 functions upstream of both the cAMP and Pmk1 signalling pathways. As a consequence, the utilization of glycogen and lipid, appressorial autophagy, actin ring formation, localization of septin proteins, as well as turgor accumulation were all affected in the Δpal1 mutant. Taken together, Pal1 regulates cAMP and the Pmk1 signalling pathway for appressorium formation and maturation to facilitate infection of M. oryzae. Endocytic protein Pal1 of Magnaporthe oryzae contributes to full pathogenicity by regulating cAMP and the Pmk1 signalling pathway for appressorium formation and maturation.
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