A thermostability perspective on enhancing physicochemical and cytological characteristics of octacalcium phosphate by doping iron and strontium.
A thermostability perspective on enhancing physicochemical and cytological characteristics of octacalcium phosphate by doping iron and strontium.
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通过掺杂铁和锶增强磷酸八钙的物理化学和细胞学特性的热稳定性观点。
DOI：
10.1016/j.bioactmat.2020.10.025
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发表时间：
2021-05
影响因子：
18.9
通讯作者：
Ye J
中科院分区：
文献类型：
--
作者：
Shi H;Ye X;Zhang J;Wu T;Yu T;Zhou C;Ye J
对其热稳定性的研究将为揭示离子掺杂对磷酸钙性能影响的机理奠定基础。本工作利用生物磷灰石的介稳前驱体磷酸八钙(OCP)作为不同离子电荷和离子半径掺杂离子(Fe3+和Sr2+)的稳定性模型。在不同温度(110~200℃)的热空气中处理后，比较研究了离子掺杂OCPs的物相、形貌、结构、物理化学性质、蛋白质亲和力、离子释放和细胞学响应。结果表明，随着温度的升高，OCP晶体逐渐崩塌，并伴随着水化层的脱水和片状晶体的解体。坍塌的晶体仍然保留了OCP的典型性质和转化为羟基磷灰石的潜力。与未掺杂的OCP相比，Fe-OCP的热稳定性较低，而锶-OCP的热稳定性较高，导致材料表面性质和离子释放不同。在相应的表面特性和早期活性离子释放的协同作用下，Fe-OCP和Sr-OCP的热稳定性显著提高了蛋白质(BSA和LSZ)的吸附以及骨髓间充质干细胞的细胞学行为(黏附、铺展、增殖和成骨分化)。这项工作为利用离子掺杂策略理解磷酸钙的改性机理和开发具有生物活性的组织修复OCP材料奠定了基础。OCP被用作不同电荷和半径的掺杂离子的稳定性模型。热处理后，OCP晶体发生崩解，结构脱水。Fe和Sr掺杂对OCP晶体的热稳定性有相反的影响。热稳定性的差异影响了OCP的表面性质和离子释放。OCP的表面活性和离子释放协同介导了其生物相容性。
Investigation of thermostability will lead the groundbreaking of unraveling the mechanism of influence of ion-doping on the properties of calcium phosphates. In this work, octacalcium phosphate (OCP), a metastable precursor of biological apatite, was used as a stability model for doping ions (Fe3+ and Sr2+) with different ionic charges and radii. After treated under hot air at different temperatures (110–200 °C), the phase, morphology, structure, physicochemical properties, protein affinity, ions release, and cytological responses of the ion-doped OCPs were investigated comparatively. The results showed that the collapse of OCP crystals gradually occurred, accompanying with the dehydration of hydrated layers and the disintegration of plate-like crystals as the temperature increased. The collapsed crystals still retained the typical properties of OCP and the potential of conversion into hydroxyapatite. Compared to the undoped OCP, Fe-OCP, and Sr-OCP had lower and higher thermostability respectively, leading to different material surface properties and ions release. The adjusted thermostability of Fe-OCP and Sr-OCP significantly enhanced the adsorption of proteins (BSA and LSZ) and the cytological behavior (adhesion, spreading, proliferation, and osteogenic differentiation) of bone marrow mesenchymal stem cells to a varying extent under the synergistic effects of corresponding surface characteristics and early active ions release. This work paves the way for understanding the modification mechanism of calcium phosphates utilizing ion doping strategy and developing bioactive OCP-based materials for tissue repair. OCP was used as a stability model for doping ions with different charges and radii. Collapse of OCP crystals occurred with structural dehydration after heat treatment. Fe and Sr doping altered the thermostability of OCP crystals in an opposite way. The thermostable difference affected the surface properties and ion release of OCP. Active surface and ion release of OCP synergistically mediated its biocompatibility.
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