Detection of atrial natriuretic peptide and its receptor in marginal cells and cochlea tissues from the developing rats.
Detection of atrial natriuretic peptide and its receptor in marginal cells and cochlea tissues from the developing rats.
复制标题
发育大鼠边缘细胞和耳蜗组织中心房钠尿肽及其受体的检测。
DOI：
--
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
目的
心房利钠肽（ANP）作为中枢神经系统的一种神经肽，调节体液和血压的稳态。为了探讨ANP在内耳中可能的生理作用，我们在原代培养的边缘细胞中检测了ANP的表达，然后我们检测了ANP及其受体（NPR-A）在不同年龄的新生和成年大鼠耳蜗组织中的表达。
方法
从新生大鼠耳蜗血管纹分离边缘细胞。培养的细胞随后通过形态学、免疫细胞化学和扫描电子显微镜（SEM）分析进行表征。应用免疫细胞化学和逆转录聚合酶链反应（RT-PCR）技术，分别检测耳蜗边缘细胞和耳蜗发育过程中心钠素（ANP）的表达。
结果
本研究显示了边缘细胞的典型特征，包括鹅卵石，多边形单层，多形性生长模式和细胞类型特异性标志物的表达，并进行了SEM分析。随后，在培养的边缘细胞中发现ANP的免疫反应产物。编码ANP和NPR-A受体的mRNA在胚胎后至生后早期在大鼠耳蜗中均有表达。在成熟期，ANP表达逐渐下调，而NPR-A受体的表达相对稳定。
结论
ANP可能由血管纹边缘细胞合成和分泌，在调节内耳微环境中起重要作用。此外，ANP可能参与耳蜗的发育和生长过程。
OBJECTIVE
Atrial natriuretic peptide (ANP) regulates the homeostasis of body fluid and blood pressure as a neuropeptide in the central nervous system. To assess the possible physiological role in the inner ear, we investigated the expression of ANP in primary culture of marginal cells, and then we detected the expression of ANP and its receptor (NPR-A) in cochlear tissues derived from neonatal and adult rats of various ages.
METHODS
Marginal cells were isolated from cochlear stria vascularis of the neonatal rats. The cultured cells were subsequently characterized by morphology, immunocytochemistry, and scanning electron microscope (SEM) analysis. In order to examine the expression of ANP in marginal cells as well as in the developing rats' cochleae, immunocytochemistry and reverse-transcription polymerase chain reaction (RT-PCR) were applied respectively.
RESULTS
The present study demonstrated typical characteristics of marginal cells, including cobblestone, polygonal monolayer, pleomorphic growth pattern and the expression of cell type-specific marker, and SEM analysis. Subsequently, immunoreactive product of ANP were found in cultured marginal cells. The mRNAs encoding ANP and NPR-A receptor was expressed in rats' cochleae from postembryonic stage to early postnatal period. During the maturation stage, ANP expression was gradually down-regulated, while the expression of NPR-A receptor was relatively stable.
CONCLUSION
ANP might be synthesized and secreted by marginal cells of stria vascularis, and could play an important role in modulating the microenvironment of the inner ear. In addition, ANP might contribute to development and growth process of cochlea.
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DOI：
10.1007/bfb0027530
发表时间：
1988
期刊：
Reviews of physiology, biochemistry and pharmacology
影响因子：
--
作者：
J. Genest;M. Cantin
通讯作者：
J. Genest;M. Cantin
影响因子：
2.8
作者：
Mikio Suzuki;T. Kitanishi;H. Kitano;Y. Yazawa;K. Kitajima;T. Takeda;Y. Tokunaga;T. Maeda;H. Kimura;I. Tooyama
通讯作者：
Mikio Suzuki;T. Kitanishi;H. Kitano;Y. Yazawa;K. Kitajima;T. Takeda;Y. Tokunaga;T. Maeda;H. Kimura;I. Tooyama
DOI：
10.2215/cjn.03780808
发表时间：
2009-02-01
影响因子：
9.8
作者：
Nigwekar, Sagar U.;Navaneethan, Sankar D.;Hix, John K.
通讯作者：
Hix, John K.
影响因子：
5.5
作者：
Quraishi, Imran H.;Raphael, Robert M.
通讯作者：
Raphael, Robert M.
NOB1在豚鼠螺旋神经节细胞中的表达。
DOI：
10.1016/j.ijporl.2008.10.028
发表时间：
2009-02-01
影响因子：
1.5
作者：
Han, Yu;Hong, Liu;Qiu, Jianhua
通讯作者：
Qiu, Jianhua