Quantum Defects as a Toolbox for the Covalent Functionalization of Carbon Nanotubes with Peptides and Proteins.
Quantum Defects as a Toolbox for the Covalent Functionalization of Carbon Nanotubes with Peptides and Proteins.
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DOI：
10.1002/anie.202003825
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发表时间：
2020-09-28
期刊：
影响因子：
--
通讯作者：
Kruss S
中科院分区：
文献类型：
--
作者：
Mann FA;Herrmann N;Opazo F;Kruss S
单壁碳纳米管 (SWCNT) 是一种一维纳米材料，在近红外（NIR，>800 nm）范围内显示荧光。过去，共价化学很少被用于功能化单壁碳纳米管，因为它会损害近红外发射。然而，碳晶格中出现了某些 sp3 缺陷（量子缺陷），它们保留了近红外荧光，甚至引入了新的红移发射峰。在这里，我们报告了使用光驱动重氮化学引入的量子缺陷，这些缺陷充当肽和蛋白质的锚点。我们证明马来酰亚胺锚允许缀合含半胱氨酸的蛋白质，例如 GFP 结合纳米抗体。此外，Fmoc 保护的苯丙氨酸缺陷可作为可见荧光团缀合的起点，以创建多色 SWCNT 并直接在纳米管上进行原位肽合成。因此，这些量子缺陷是一个通用平台，可以调整纳米管的光物理性质及其表面化学性质。 两种新的量子缺陷被纳入带有锚定基团的单壁碳纳米管（SWCNT）中，用于生物分子的功能化。这种方法的潜力和多功能性通过 GFP 结合纳米体的共轭以及（荧光）肽链直接在纳米管碳晶格上的生长得到了证明。
Single‐walled carbon nanotubes (SWCNTs) are a 1D nanomaterial that shows fluorescence in the near‐infrared (NIR, >800 nm). In the past, covalent chemistry was less explored to functionalize SWCNTs as it impairs NIR emission. However, certain sp3 defects (quantum defects) in the carbon lattice have emerged that preserve NIR fluorescence and even introduce a new, red‐shifted emission peak. Here, we report on quantum defects, introduced using light‐driven diazonium chemistry, that serve as anchor points for peptides and proteins. We show that maleimide anchors allow conjugation of cysteine‐containing proteins such as a GFP‐binding nanobody. In addition, an Fmoc‐protected phenylalanine defect serves as a starting point for conjugation of visible fluorophores to create multicolor SWCNTs and in situ peptide synthesis directly on the nanotube. Therefore, these quantum defects are a versatile platform to tailor both the nanotube's photophysical properties as well as their surface chemistry. Two new quantum defects were incorporated into single‐walled carbon nanotubes (SWCNT) carrying anchor groups for functionalization with biomolecules. The potential and versatility of this approach was demonstrated by the conjugation of a GFP‐binding nanobody as well as the growth of (fluorescent) peptide chains directly on the nanotube's carbon lattice.
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DOI：
10.1083/jcb.201409074
发表时间：
2015-06-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Helma J;Cardoso MC;Muyldermans S;Leonhardt H
通讯作者：
Leonhardt H
影响因子：
10.8
作者：
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影响因子：
13.6
作者：
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Cognet, Laurent
影响因子：
17.1
作者：
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通讯作者：
Heller DA
影响因子：
4.9
作者：
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通讯作者：
Prato, M