Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects.
Analysis of the Total Biflavonoids Extract from Selaginella doederleinii by HPLC-QTOF-MS and Its In Vitro and In Vivo Anticancer Effects.
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DOI：
10.3390/molecules22020325
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发表时间：
2017-02-20
期刊：
影响因子：
--
通讯作者：
Lin X
中科院分区：
文献类型：
--
作者：
Yao H;Chen B;Zhang Y;Ou H;Li Y;Li S;Shi P;Lin X
卷柏（Selaginella doederleinii Hieron）是中国传统的民间抗肿瘤草药。本文采用高效液相色谱-电喷雾四极杆飞行时间质谱联用（HPLC-ESI-QTOF MS/MS）负离子模式对菟菟子总黄酮提取物的植物化学成分进行了研究，并对其体外和体内抗癌作用进行了评价。采用QTOF质谱/质谱法对杜氏松中IC3′-IIC8′、IC3′-IIC6′、IC3′-IIC3′和C-O连接的4种生物黄酮进行了初步鉴定。首次报道了IC3 ' -IIC3 ‘ ’连接的生物黄酮的断裂行为。共鉴定或初步鉴定了20种黄酮类化合物，其中8种为首次从菟丝子中分离得到。此外，3-（4,5-二甲基-2-噻唑基）-2,5-二苯基溴化太他唑（MTT）实验和雄性C57BL/6小鼠lewis肺癌（LLC）异种移植模型显示，菟丝子总黄酮提取物具有良好的抗癌作用。本研究结果可为植物样品中生物类黄酮的鉴定提供有用的知识，并进一步了解该植物的化学预防功能。
Selaginella doederleinii Hieron has been traditionally used as a folk antitumor herbal medicine in China. In this paper, the phytochemical components of the total biflavonoids extract from S. doederleinii were studied by using high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF MS/MS) in negative ion mode, and their in vitro and in vivo anticancer effects were evaluated. Four types of biflavonoids from S. doederleinii, including IC3′–IIC8′′, IC3′–IIC6′′, IC3′–IIC3′′′, and C–O linked biflavonoids were examined originally using QTOF MS/MS. The fragmentation behavior of IC3′–IIC3′′′ linked biflavonoids was reported for the first time. A total of twenty biflavonoids were identified or tentatively characterized and eight biflavonoids were found from S. doederleinii for the first time. Furthermore, the 3-(4,5-Dimethyl-2-thizolyl)-2,5-diphenyltertazolium bromide (MTT) assay and xenograft model of mouse lewis lung cancer(LLC) in male C57BL/6 mice revealed favorable anticancer properties of the total biflavonoids extracts from S. doederleinii. The results of this work could provide useful knowledge for the identification of biflavonoids in herbal samples and further insights into the chemopreventive function of this plant.
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