Autonomous plasmid-like replication of a conjugative transposon.
Autonomous plasmid-like replication of a conjugative transposon.
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DOI：
10.1111/j.1365-2958.2009.06985.x
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发表时间：
2010-01
影响因子：
3.6
通讯作者：
Grossman AD
中科院分区：
文献类型：
--
作者：
Lee CA;Babic A;Grossman AD
整合元素和共轭元素(ICE)，也称为接合转座子是一种可移动的遗传元件，参与了许多生物学过程，包括致病、共生和抗生素耐药性的传播。与染色体外自主复制的接合质粒不同，ICES整合在染色体中，在染色体复制过程中被动复制。人们普遍认为，冰不会自主复制。我们发现，当被诱导时，枯草芽孢杆菌ICEBs1经历了类似质粒的自主复制。复制是单向的，从ICEBs1的转移源ORIT开始，需要ICEBs1编码的松弛酶缺口。复制还需要染色体复制所需的几种宿主蛋白，但不需要复制解旋酶DNAC或解旋酶加载蛋白DNAB。相反，ICEBs1的复制需要解旋酶PcrA，而解旋酶PcrA是许多质粒滚动循环复制所必需的。从供体转移ICEBs1需要PcrA，但不需要复制，这表明PcrA而不是DNA复制促进了ICEBs1 DNA的水平转移。虽然水平转移不需要ICEBs1的复制，但为了元件的稳定性需要ICEBs1的复制。我们认为，自主的质粒状复制是ICS的共同属性，有助于这些可移动遗传元件在细菌种群中的稳定和维持。
Integrative and conjugative elements (ICEs), a.k.a. conjugative transposons, are mobile genetic elements involved in many biological processes, including pathogenesis, symbiosis, and the spread of antibiotic resistance. Unlike conjugative plasmids that are extra-chromosomal and replicate autonomously, ICEs are integrated in the chromosome and replicate passively during chromosomal replication. It is generally thought that ICEs do not replicate autonomously. We found that when induced, Bacillus subtilis ICEBs1 undergoes autonomous plasmid-like replication. Replication was unidirectional, initiated from the ICEBs1 origin of transfer, oriT, and required the ICEBs1-encoded relaxase NicK. Replication also required several host proteins needed for chromosomal replication, but did not require the replicative helicase DnaC or the helicase loader protein DnaB. Rather, replication of ICEBs1 required the helicase PcrA that is required for rolling circle replication of many plasmids. Transfer of ICEBs1 from the donor required PcrA, but did not require replication, indicating that PcrA, and not DNA replication, facilitates unwinding of ICEBs1 DNA for horizontal transfer. Although not needed for horizontal transfer, replication of ICEBs1 was needed for stability of the element. We propose that autonomous plasmid-like replication is a common property of ICEs and contributes to the stability and maintenance of these mobile genetic elements in bacterial populations.
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