Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding.
Sialotranscriptomics of Rhipicephalus zambeziensis reveals intricate expression profiles of secretory proteins and suggests tight temporal transcriptional regulation during blood-feeding.
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DOI：
10.1186/s13071-017-2312-4
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发表时间：
2017-08-10
影响因子：
3.2
通讯作者：
Mans BJ
中科院分区：
文献类型：
--
作者：
de Castro MH;de Klerk D;Pienaar R;Rees DJG;Mans BJ
壁虱分泌多种不同的分泌蛋白进入宿主体内，以逃避其免疫反应并促进血液摄取，使分泌蛋白成为生产重组抗蜱疫苗的有吸引力的靶标。在南部非洲，被广泛忽视的扁虱是微小泰勒虫(Theileria Parva)的有效媒介，但其现有序列信息有限。下一代测序在最近几年提高了扁虱的序列可用性，并帮助确定了分泌蛋白的特征。本研究主要研究了赞比西河鼠唾液腺转录组的从头组装和注释以及分泌蛋白转录物在雌性和雄性硬蜱开始摄食前以及摄食早期和后期的时间表达。赞比西山核桃的唾液酸转录组产生了23,631个转录本，其中预测了13,584个非冗余蛋白。其中86%含有预测的起始和终止密码子，估计为推测的全长蛋白质。五分之一(2569个)的预测蛋白被注释为推测的分泌蛋白，并解释了转录组中52%的表达。表达分析表明，2832个转录本在取食时间点之间存在差异，1209个转录本在不同性别之间存在差异。表达分析进一步表明，57%的注释分泌蛋白转录本是差异表达的。观察了雌性硬蜱摄食过程中分泌蛋白转录本的动态表达谱。因此，在早期取食期间，可能是为了建立取食部位，一些转录本上调，然后在后期取食，其中52%被下调，这表明在特定的取食阶段需要转录本。这表明分泌蛋白处于严格的转录调控之下，在进食过程中微调它们在唾液腺中的表达。7947个转录本没有预测到开放阅读框。这一类代表了17%的差异表达的转录本，这表明长的非编码RNA在蜱类取血中具有潜在的转录调节功能。组装的唾液酸转录组极大地扩大了赞比西河鼠的序列可获得性，有助于我们理解血液摄食过程中分泌蛋白的转录，将成为未来疫苗候选选择的宝贵资源。本文的在线版本(doi：10.1186/s13071-0172312-4)包含补充材料，可供授权用户使用。
Ticks secrete a diverse mixture of secretory proteins into the host to evade its immune response and facilitate blood-feeding, making secretory proteins attractive targets for the production of recombinant anti-tick vaccines. The largely neglected tick species, Rhipicephalus zambeziensis, is an efficient vector of Theileria parva in southern Africa but its available sequence information is limited. Next generation sequencing has advanced sequence availability for ticks in recent years and has assisted the characterisation of secretory proteins. This study focused on the de novo assembly and annotation of the salivary gland transcriptome of R. zambeziensis and the temporal expression of secretory protein transcripts in female and male ticks, before the onset of feeding and during early and late feeding. The sialotranscriptome of R. zambeziensis yielded 23,631 transcripts from which 13,584 non-redundant proteins were predicted. Eighty-six percent of these contained a predicted start and stop codon and were estimated to be putatively full-length proteins. A fifth (2569) of the predicted proteins were annotated as putative secretory proteins and explained 52% of the expression in the transcriptome. Expression analyses revealed that 2832 transcripts were differentially expressed among feeding time points and 1209 between the tick sexes. The expression analyses further indicated that 57% of the annotated secretory protein transcripts were differentially expressed. Dynamic expression profiles of secretory protein transcripts were observed during feeding of female ticks. Whereby a number of transcripts were upregulated during early feeding, presumably for feeding site establishment and then during late feeding, 52% of these were downregulated, indicating that transcripts were required at specific feeding stages. This suggested that secretory proteins are under stringent transcriptional regulation that fine-tunes their expression in salivary glands during feeding. No open reading frames were predicted for 7947 transcripts. This class represented 17% of the differentially expressed transcripts, suggesting a potential transcriptional regulatory function of long non-coding RNA in tick blood-feeding. The assembled sialotranscriptome greatly expands the sequence availability of R. zambeziensis, assists in our understanding of the transcription of secretory proteins during blood-feeding and will be a valuable resource for future vaccine candidate selection. The online version of this article (doi:10.1186/s13071-017-2312-4) contains supplementary material, which is available to authorized users.
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影响因子：
6.7
作者：
Dai J;Narasimhan S;Zhang L;Liu L;Wang P;Fikrig E
通讯作者：
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影响因子：
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DOI：
10.1038/nrmicro2145
发表时间：
2009-07
期刊：
Nature reviews. Microbiology
影响因子：
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通讯作者：
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影响因子：
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