Bluetongue virus infection in naïve cattle: Identification of circulating serotypes and associated Culicoides biting midge species in Trinidad.
Bluetongue virus infection in naïve cattle: Identification of circulating serotypes and associated Culicoides biting midge species in Trinidad.
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DOI：
10.1016/j.vetmic.2017.09.008
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发表时间：
2017-11
影响因子：
3.3
通讯作者：
Oura CAL
中科院分区：
文献类型：
--
作者：
Brown-Joseph T;Batten C;Harrup LE;Frost L;Flannery J;Hicks H;Ramkissoon V;Ramdeen R;Carrington CV;Oura CAL
蓝舌病毒在特立尼达的高流行。特立尼达6种蓝舌病毒血清型的鉴定。在特立尼达首次分离到BTV-2和BTV-5。出现重组病毒的风险。为了更好地了解与交易牛有关的风险，重要的是要知道哪些血清型的蓝舌病毒(BTV)在出口国和进口国传播。因此，本研究旨在确定特立尼达BTV的流行血清型。从60头BTV-naive进口牛抵达该国后的6个月内每月采集血样。对样本进行病毒学(PCR和病毒分离)和血清学(ELISA)分析，并在牛舍附近放置CDC灯光诱捕器，以诱捕和鉴定存在的叮咬库蚊的种类。所有的牛在抵达该国后的三个月内都进行了BTV抗体的血清转换，并在整个研究剩余的时间里用实时逆转录聚合酶链式反应(RRT-PCR)检测了样本中的BTV-RNA。在牛身上观察到的感染模式表明，连续感染具有多种血清型。对原始血样进行BTV血清型特异性RRT-PCR和病毒分离，然后对所选样本进行血清型特异性RRT-PCR，证实了BTV血清型1、2、3、5、12和17型的存在。这是特立尼达首次报道BTV-2和BTV-5。光吸引器放置在靠近牛的地方，主要诱捕了1913种库蚊(96%)，另有6种库蚊占诱捕样本的4%。多种BTV血清型在特立尼达的传播凸显了定期监测的必要性，这将有助于对牲畜贸易进行风险评估。
High prevalence of bluetongue virus in Trinidad. Identification of six bluetongue virus serotypes in Trinidad. First isolation of BTV-2 and BTV-5 in Trinidad. Risk of emergence of reassortant viruses. To better understand risks associated with trading cattle, it is important to know which serotypes of Bluetongue virus (BTV) are circulating within the exporting and importing country. Hence, this study was conducted to identify the circulating serotypes of BTV in Trinidad. Blood samples were collected monthly from sixty BTV- naïve imported cattle over a six month period after their arrival in the country. Virological (PCR and virus isolation) and serological (ELISA) analyses were carried out on the samples and CDC light traps were placed near the cattle enclosure to trap and identify the species of Culicoides biting midges that were present. All of the cattle seroconverted for BTV antibodies within three months of their arrival in the country and real-time reverse transcription PCR (rRT-PCR) detected BTV-RNA in the samples throughout the remainder of the study. The patterns of infection observed in the cattle indicated serial infections with multiple serotypes. A combination of BTV serotype-specific rRT-PCR on the original blood samples and virus isolation followed by serotype-specific rRT-PCR on selected samples, confirmed the presence of BTV serotypes 1, 2, 3, 5, 12 and 17. This is the first report of BTV-2 and BTV-5 in Trinidad. Light-suction traps placed in close proximity to the cattle predominantly trapped Culicoides insignis Lutz 1913 species (96%), with a further six Culicoides species making up the remaining 4% of trapped samples. The circulation of multiple BTV serotypes in Trinidad underlines the need for regular surveillance, which will contribute to the development of risk assessments for trade in livestock.
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影响因子：
6.7
作者：
Nomikou K;Hughes J;Wash R;Kellam P;Breard E;Zientara S;Palmarini M;Biek R;Mertens P
通讯作者：
Mertens P
影响因子：
5.4
作者：
Caporale M;Di Gialleonorado L;Janowicz A;Wilkie G;Shaw A;Savini G;Van Rijn PA;Mertens P;Di Ventura M;Palmarini M
通讯作者：
Palmarini M
影响因子：
3.3
作者：
TANYA, VN;GREINER, EC;GIBBS, EPJ
通讯作者：
GIBBS, EPJ
影响因子：
2.4
作者：
Di Gialleonardo, L.;Migliaccio, P.;Savini, G.
通讯作者：
Savini, G.
影响因子：
3.3
作者：
Bonneau, KR;DeMaula, CD;MacLachlan, NJ
通讯作者：
MacLachlan, NJ