Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury.
Exogenous platelet-derived growth factor improves neurovascular unit recovery after spinal cord injury.
复制标题
外源性血小板衍生生长因子可改善脊髓损伤后神经血管单元的恢复。
DOI：
10.4103/1673-5374.295347
复制
发表时间：
2021-04
影响因子：
6.1
通讯作者：
Zhang HY
中科院分区：
文献类型：
--
作者：
Ye LX;An NC;Huang P;Li DH;Zheng ZL;Ji H;Li H;Chen DQ;Wu YQ;Xiao J;Xu K;Li XK;Zhang HY
脊髓屏障在脊髓损伤后的恢复中起着至关重要的作用。在体外，通过暴露于200μm建立人脐静脉内皮细胞在受伤前2小时，用5 ng/ml血小板衍生的蛋白质的表达，包括咬合蛋白，Claudin 5和β-catenin的表达显着降低了，在体外的增长效果显着降低，H2O2。公斤氯喹，一种自噬抑制剂，在脊髓损伤之前成功进行了3天，我们的发现表明，血小板衍生的生长因子可以通过调节自噬来促进内皮细胞的修复，改善脊髓屏障的功能，并促进脊髓疾病的掌握委员会的恢复。 No.WYDW2018-0043）于2018年7月。
The blood-spinal cord barrier plays a vital role in recovery after spinal cord injury. The neurovascular unit concept emphasizes the relationship between nerves and vessels in the brain, while the effect of the blood-spinal cord barrier on the neurovascular unit is rarely reported in spinal cord injury studies. Mouse models of spinal cord injury were established by heavy object impact and then immediately injected with platelet-derived growth factor (80 μg/kg) at the injury site. Our results showed that after platelet-derived growth factor administration, spinal cord injury, neuronal apoptosis, and blood-spinal cord barrier permeability were reduced, excessive astrocyte proliferation and the autophagy-related apoptosis signaling pathway were inhibited, collagen synthesis was increased, and mouse locomotor function was improved. In vitro, human umbilical vein endothelial cells were established by exposure to 200 μM H2O2. At 2 hours prior to injury, in vitro cell models were treated with 5 ng/mL platelet-derived growth factor. Our results showed that expression of blood-spinal cord barrier-related proteins, including Occludin, Claudin 5, and β-catenin, was significantly decreased and autophagy was significantly reduced. Additionally, the protective effects of platelet-derived growth factor could be reversed by intraperitoneal injection of 80 mg/kg chloroquine, an autophagy inhibitor, for 3 successive days prior to spinal cord injury. Our findings suggest that platelet-derived growth factor can promote endothelial cell repair by regulating autophagy, improve the function of the blood-spinal cord barrier, and promote the recovery of locomotor function post-spinal cord injury. Approval for animal experiments was obtained from the Animal Ethics Committee, Wenzhou Medical University, China (approval No. wydw2018-0043) in July 2018.
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DOI：
10.1007/978-3-540-78281-0_7
发表时间：
2010-01-01
期刊：
ANGIOGENESIS INHIBITION
影响因子：
--
作者：
Hellberg, Carina;Ostman, Arne;Heldin, C. -H.
通讯作者：
Heldin, C. -H.
影响因子：
4.7
作者：
Lee, Jee Y.;Kim, Hwang S.;Yune, Tae Y.
通讯作者：
Yune, Tae Y.
成纤维细胞生长因子 21 通过抑制氧化损伤和自噬性细胞死亡来促进周围神经再生。
影响因子：
5.3
作者：
Lu Y;Li R;Zhu J;Wu Y;Li D;Dong L;Li Y;Wen X;Yu F;Zhang H;Ni X;Du S;Li X;Xiao J;Wang J
通讯作者：
Wang J
影响因子：
11.1
作者：
del Zoppo GJ
通讯作者：
del Zoppo GJ
影响因子：
56.9
作者：
KECK, PJ;HAUSER, SD;CONNOLLY, DT
通讯作者：
CONNOLLY, DT