Evaluation of a Liposome System for the Delivery of Desferrioxamine to Lungs in Rats
Evaluation of a Liposome System for the Delivery of Desferrioxamine to Lungs in Rats
复制标题
用于将去铁胺递送至大鼠肺的脂质体系统的评价
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
U. Taitelman
中科院分区：
文献类型：
--
作者：
A. Tabak;E. Hoffer;U. Taitelman
摘要-通过两种不同的技术制备的不同脂质成分和大小的脂质体，评估了它们将去铁胺输送到肺内作为抗氧化性肺损伤的治疗的潜力。从含有二棕榈酰磷脂酰胆碱、硬脂胺、胆固醇和维生素E的脂质混合物中制备了多层囊泡和反向蒸发囊泡。与游离去铁胺相比，静脉注射去铁胺脂质体(10 0 mg kg−1)可显著延长去铁胺在所有受试器官中的存在时间。通过2μm聚碳酸酯薄膜挤出的反向蒸发微囊注射，与其他类型的脂质体相比，去铁胺和脂质体维生素E在肺内的滞留时间更长。对支气管肺泡灌洗液和肺泡巨噬细胞中脂质体成分的检测表明，在给药后3h和17h，该技术可回收去铁胺剂量的7×10−3%。肺泡腔中的高残留浓度证实了这样的假设，即脂质体被包裹在肺泡巨噬细胞中时可以被输送到肺组织。
Abstract— Liposomes with various lipid composition and sizes, prepared by two different techniques were evaluated for their potential to deliver desferrioxamine to lungs as a treatment against oxidative lung damage. Multilamellar vesicles (MLV) and reverse evaporation vesicles were prepared out of a lipid mixture containing dipalmitoyl phosphatidylcholine, stearyl amine, cholesterol and vitamin E. The administration of desferrioxamine‐encapsulated liposomes to rats by the intravenous route at a dose of 100 mg kg−1, significantly prolonged the presence of desferrioxamine in all the tested organs when compared with the administration of free desferrioxamine. The injection of reverse evaporation vesicles extruded through a 2 μm polycarbonate membrane exhibited a longer residence time of the desferrioxamine and of liposomal vitamin E in lungs compared with the other types of liposomes tested. The examination of liposome components in the bronchoalveolar lavage fluid (BALF) and the alveolar macrophages recovered from BALF revealed that about 7 × 10−3% of the administered desferrioxamine dose was recovered by this technique at 3 and 17 h after liposome administration. This high residual concentration in the alveolar space confirms the hypothesis that liposomes can be delivered to the lung tissue when encapsulated in alveolar macrophages.
百草枯对犬支气管肺泡灌洗液的影响。
影响因子：
3.8
作者：
Hampson,EC;Eyles,DW;Pond,SM
通讯作者：
Pond,SM
通过气管内施用脂质体封装的超氧化物歧化酶或过氧化氢酶来防止大鼠的肺氧中毒。
DOI：
10.1164/arrd.1985.132.1.164
发表时间：
1985
期刊：
The American review of respiratory disease
影响因子：
--
作者：
Padmanabhan,RV;Gudapaty,R;Liener,IE;Schwartz,BA;Hoidal,JR
通讯作者：
Hoidal,JR