Direct ex vivo simian immunodeficiency virus (SIV)-specific cytotoxic activity detected from small intestine intraepithelial lymphocytes of SIV-infected macaques at an advanced stage of infection
Direct ex vivo simian immunodeficiency virus (SIV)-specific cytotoxic activity detected from small intestine intraepithelial lymphocytes of SIV-infected macaques at an advanced stage of infection
复制标题
从感染晚期 SIV 感染的猕猴的小肠上皮内淋巴细胞中检测到直接离体猿猴免疫缺陷病毒 (SIV) 特异性细胞毒活性
作者：
A. Couëdel;R. Le Grand;M. Tulliez;J. Guillet;A. Venet
人类免疫缺陷病毒（HIV）在感染末期引起淋巴组织的严重紊乱，免疫系统明显异常。由于消化黏膜免疫系统是迄今为止人体最大的淋巴器官，我们试图在SIV-猕猴HIV感染模型中评估其在猿猴免疫缺陷病毒（SIV）感染晚期的功能活性。研究人员对两只长期静脉注射siv感染的猕猴进行了研究，其中一只处于艾滋病阶段。对肠上皮内淋巴细胞（IEL）进行分离、分析，并与血液、脾脏和不同淋巴结的淋巴细胞进行比较：IEL主要是CD8+ T淋巴细胞，表达α - β - 7整合素，缺乏CD28共激活分子。直接离体siv特异性细胞毒活性在两种猕猴的IEL中都明显存在，而在其他部位则较弱或不存在。据我们所知，这是在siv感染的猕猴的小肠IEL中首次报道siv特异性细胞毒活性。考虑到siv -猕猴模型与人类HIV感染的高度相似性，这些结果可能对HIV感染的发病机制非常重要，更普遍的是对消化CD8+ IEL群体的特征和功能非常重要。
Human immunodeficiency virus (HIV) induces a profound disorganization of the lymphoid tissues with marked abnormalities of the immune system at the terminal stage of infection. Since the digestive mucosal immune system is by far the largest lymphoid organ of the body, we attempted to evaluate its functional activity in advanced stages of simian immunodeficiency virus (SIV) infection in the SIV-macaque model of HIV infection. Two chronically intravenously SIV-infected macaques, including one at the AIDS stage, were studied. Intestinal intraepithelial lymphocytes (IEL) were isolated, analyzed, and compared to lymphocytes obtained from blood, spleen, and different lymph nodes: IEL were predominantly CD8+ T lymphocytes expressing the alphaE beta7 integrin and lacking the CD28 coactivatory molecule. A direct ex vivo SIV-specific cytotoxic activity was prominently found in the IEL of both macaques and was weaker or absent in the other sites. To our knowledge, this is the first report of SIV-specific cytotoxic activity from small intestine IEL in SIV-infected macaques. Considering the high similitude of the SIV-macaque model with the HIV infection in humans, these results may be highly important for the pathogenesis of HIV infection and more generally important for the characterization and function of digestive CD8+ IEL population.
登录
查看更多内容
SIVsmmPBj14 诱导猕猴淋巴细胞上粘膜整合素的表达。
DOI：
10.1006/viro.1996.0010
发表时间：
1996
期刊：
Virology.
影响因子：
--
作者：
Gummuluru,S;Novembre,FJ;Seshi,B;Dewhurst,S
通讯作者：
Dewhurst,S
影响因子：
4.4
作者：
A. Hamann;D. Andrew;D. Jablonski‐Westrich;B. Holzmann;E. Butcher
通讯作者：
A. Hamann;D. Andrew;D. Jablonski‐Westrich;B. Holzmann;E. Butcher
影响因子：
4.4
作者：
J. Monteiro;F. Batliwalla;Harry Ostrer;Peter K. Gregersen
通讯作者：
J. Monteiro;F. Batliwalla;Harry Ostrer;Peter K. Gregersen
影响因子：
20.3
作者：
T. Carlos;J. Harlan
通讯作者：
T. Carlos;J. Harlan
一种急性致命的猿猴免疫缺陷病毒会刺激表达 V beta 7 和 V beta 14 的 T 淋巴细胞的扩增。
DOI：
10.1073/pnas.91.16.7501
发表时间：
1994
影响因子：
11.1
作者：
Chen,ZW;Kou,ZC;Shen,L;Regan,JD;Lord,CI;Halloran,M;Lee-Parritz,D;Fultz,PN;Letvin,NL
通讯作者：
Letvin,NL