Systematic analysis of sequences and expression patterns of drought-responsive members of the HD-Zip gene family in maize.
Systematic analysis of sequences and expression patterns of drought-responsive members of the HD-Zip gene family in maize.
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DOI：
10.1371/journal.pone.0028488
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Cheng B
中科院分区：
文献类型：
--
作者：
Zhao Y;Zhou Y;Jiang H;Li X;Gan D;Peng X;Zhu S;Cheng B
同源结构域亮氨酸拉链（HD-Zip）基因家族成员编码植物特有的转录因子，并且在植物生长和发育中具有多种功能，例如各种胁迫反应、器官形成和维管发育。虽然这个家族的系统表征已经在拟南芥和水稻中进行，但对玉米（Zea mays L.）中的HD-Zip基因知之甚少。在这项研究中，我们描述了玉米基因组中HD-Zip基因的鉴定和结构特征。使用Blast搜索工具在玉米基因组中鉴定了55个HD-Zip基因（Zmhdz 1 -55）的完整集合，并基于遗传学将其分为四类（HD-Zip I-IV）。这些基因的染色体定位显示，它们在所有10条染色体上分布不均匀。片段重复主要是玉米HD-ZIP基因家族的扩增，而串联重复只负责HD-Zip II基因的扩增。此外，大多数的玉米HD-Zip I基因被发现含有过多的压力相关的顺式元件在其启动子序列。采用实时荧光定量PCR（qRT-PCR）技术分析了17个HD-Zip I基因在干旱胁迫下的表达水平。17个玉米HD-ZIP I基因均受干旱胁迫的调控，且在同一对姐妹中重复的基因表达模式相似，表明它们在进化过程中具有保守的功能。我们的研究结果揭示了玉米HD-Zip基因家族的全面概述，并为Zmhdz基因的克隆和功能研究提供了选择的第一步，以揭示它们在玉米生长和发育中的作用。
Members of the homeodomain-leucine zipper (HD-Zip) gene family encode transcription factors that are unique to plants and have diverse functions in plant growth and development such as various stress responses, organ formation and vascular development. Although systematic characterization of this family has been carried out in Arabidopsis and rice, little is known about HD-Zip genes in maize (Zea mays L.). In this study, we described the identification and structural characterization of HD-Zip genes in the maize genome. A complete set of 55 HD-Zip genes (Zmhdz1-55) were identified in the maize genome using Blast search tools and categorized into four classes (HD-Zip I-IV) based on phylogeny. Chromosomal location of these genes revealed that they are distributed unevenly across all 10 chromosomes. Segmental duplication contributed largely to the expansion of the maize HD-ZIP gene family, while tandem duplication was only responsible for the amplification of the HD-Zip II genes. Furthermore, most of the maize HD-Zip I genes were found to contain an overabundance of stress-related cis-elements in their promoter sequences. The expression levels of the 17 HD-Zip I genes under drought stress were also investigated by quantitative real-time PCR (qRT-PCR). All of the 17 maize HD-ZIP I genes were found to be regulated by drought stress, and the duplicated genes within a sister pair exhibited the similar expression patterns, suggesting their conserved functions during the process of evolution. Our results reveal a comprehensive overview of the maize HD-Zip gene family and provide the first step towards the selection of Zmhdz genes for cloning and functional research to uncover their roles in maize growth and development.
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影响因子：
7
作者：
Blanc, G;Hokamp, K;Wolfe, KH
通讯作者：
Wolfe, KH
Pfam：氏族、网络工具和服务。
影响因子：
14.9
作者：
Finn, Robert D.;Mistry, Jaina;Schuster-Bockler, Benjamin;Griffiths-Jones, Sam;Hollich, Volker;Lassmann, Timo;Moxon, Simon;Marshall, Mhairi;Khanna, Ajay;Durbin, Richard;Eddy, Sean R.;Sonnhammer, Erik L. L.;Bateman, Alex
通讯作者：
Bateman, Alex
影响因子：
5.4
作者：
Jain, Mukesh;Tyagi, Akhilesh K.;Khurana, Jitendra P.
通讯作者：
Khurana, Jitendra P.
影响因子：
7.4
作者：
Baima, S;Possenti, M;Morelli, G
通讯作者：
Morelli, G
DOI：
10.1006/bbrc.2001.4904
发表时间：
2001-06-01
影响因子：
3.1
作者：
Lee, YH;Oh, HS;Chun, JY
通讯作者：
Chun, JY