Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.
Live cell imaging reveals differential modifications to cytoplasmic dynein properties by phospho- and dephosphomimic mutations of the intermediate chain 2C S84.
复制标题
DOI：
10.1002/jnr.23388
复制
发表时间：
2014-09
影响因子：
4.2
通讯作者：
Pfister, K. Kevin
中科院分区：
文献类型：
--
作者：
Blasier, Kiev R.;Humsi, Michael K.;Ha, Junghoon;Ross, Mitchell W.;Smiley, W. Russell;Inamdar, Nirja A.;Mitchell, David J.;Lo, Kevin W-H.;Pfister, K. Kevin
Cytoplasmic dynein is a multi-subunit motor protein responsible for intracellular cargo transport toward microtubule minus ends. There are multiple isoforms of the dynein intermediate chain (DYNC1I, IC) which is encoded by two genes. One way to regulate cytoplasmic dynein is by IC phosphorylation. The IC-2C isoform is expressed in all cells and the functional significance of phosphorylation on IC-2C serine 84 was investigated using live cell imaging of fluorescent protein-tagged wild type IC-2C (WT) and phospho- and dephospho-mimic mutant isoforms in axonal transport model systems. Both mutations modulated dynein functional properties. The dephospho-mimic mutant IC-2C S84A had greater co-localization with mitochondria than IC-2C wild-type (WT) or the phospho-mimic mutant IC-2C S84D. The dephospho-mimic mutant IC-2C S84A was also more likely to be motile than the phospho-mimic mutant IC-2C S84D or IC-2C WT. In contrast, the phospho-mimic mutant IC-2C S84D mutant was more likely to move in the retrograde direction than was the IC-2C S84A mutant. The phospho-mimic IC-2C S84D was also as likely as IC-2C WT to co-localize with mitochondria. Both the S84D phospho- and S84A, dephospho-mimic mutants were found to be capable of microtubule minus end directed (retrograde) movement in axons. They were also observed to be passively transported in the anterograde direction. These data suggest that the IC-2C S84 has a role in modulating dynein properties.
登录
查看更多内容
DOI：
10.1083/jcb.200908075
发表时间：
2009-12-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ally S;Larson AG;Barlan K;Rice SE;Gelfand VI
通讯作者：
Gelfand VI
DOI：
10.1038/nrm2804
发表时间：
2009-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1523/jneurosci.5599-11.2012
发表时间：
2012-10-31
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Mitchell DJ;Blasier KR;Jeffery ED;Ross MW;Pullikuth AK;Suo D;Park J;Smiley WR;Lo KW;Shabanowitz J;Deppmann CD;Trinidad JC;Hunt DF;Catling AD;Pfister KK
通讯作者：
Pfister KK
DOI：
10.1016/j.bbrc.2008.05.008
发表时间：
2008-08-01
影响因子：
3.1
作者：
Abe, Takako K.;Honda, Takao;Kuwano, Ryozo
通讯作者：
Kuwano, Ryozo
影响因子：
5.3
作者：
Grabham, Peter W.;Seale, Garrett E.;Vallee, Richard B.
通讯作者：
Vallee, Richard B.