Expression domains of a zebrafish homologue of the Drosophila pair-rule gene hairy correspond to primordia of alternating somites.
Expression domains of a zebrafish homologue of the Drosophila pair-rule gene hairy correspond to primordia of alternating somites.
复制标题
果蝇配对规则基因毛状的斑马鱼同源物的表达域对应于交替体节的原基。
作者：
Marcus Müller;Elisabeth v. Weizsäcker;J. Campos
her1 是编码 bHLH 蛋白的斑马鱼 cDNA，具有果蝇 HAIRY-E(SPL) 家族成员的所有特征。在原肠胚形成晚期，her1 在外线边缘和外线前沿后面的下胚层细胞的两个不同横带中表达。外胚层形成完成后，这种模式在原肠胚形成后阶段基本保持不变。边缘结构域并入尾芽中，并且根据时间点，两对或三对表达细胞带存在于轴旁前体中胚层中，并被缺乏转录物的区域分隔开。用荧光素-右旋糖酐标记 her1 表达域内的细胞表明，外缘边缘和尾芽中的细胞并未分配给特定的体节。然而，细胞向体节的分配发生在边缘表达域和其前面的第一个表达带之间。此外，her1 带和中间的非表达区各自代表体节的原基。这种表达模式与果蝇配对规则基因的表达模式非常相似。讨论了 her1 可能参与与体节形成相关的功能。
her1 is a zebrafish cDNA encoding a bHLH protein with all features characteristic of members of the Drosophila HAIRY-E(SPL) family. During late gastrulation stages, her1 is expressed in the epibolic margin and in two distinct transverse bands of hypoblastic cells behind the epibolic front. After completion of epiboly, this pattern persists essentially unchanged through postgastrulation stages; the marginal domain is incorporated in the tail bud and, depending on the time point, either two or three paired bands of expressing cells are present within the paraxial presomitic mesoderm separated by regions devoid of transcripts. Labelling of cells within the her1 expression domains with fluorescein-dextran shows that the cells in the epibolic margin and the tail bud are not allocated to particular somites. However, allocation of cells to somites occurs between the marginal expression domain and the first expression band, anterior to it. Moreover, the her1 bands, and the intervening non-expressing zones, each represents the primordium of a somite. This expression pattern is highly reminiscent of that of Drosophila pair-rule genes. A possible participation of her1 in functions related to somite formation is discussed.
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