Electrophysiological Properties of Genetically Identified Histaminergic Neurons.
Electrophysiological Properties of Genetically Identified Histaminergic Neurons.
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DOI：
10.1016/j.neuroscience.2020.06.031
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发表时间：
2020-09-15
期刊：
影响因子：
3.3
通讯作者：
Elmquist JK
中科院分区：
文献类型：
--
作者：
Michael NJ;Zigman JM;Williams KW;Elmquist JK
结节乳头核（TMN）的组胺能神经元是行为和体内平衡过程的重要调节因子。先前的研究表明，组胺能神经元表现出一种特殊的电生理特征，允许在脑切片制备中识别它们。然而，这些先前的研究主要集中在大鼠颞叶网络腹侧亚区的神经元上。因此，尚不清楚这种电生理特性是否延伸到小鼠，包括TMN的其他亚区，以及雄性和雌性之间的潜在差异。为了进一步表征组胺能神经元的电生理特性，我们对表达Cre重组酶的转基因小鼠在表达组氨酸脱羧酶（HDC）的细胞中进行了全细胞膜片钳记录；唯一的组胺合成酶（Hdc-cre::tdTomato）。尽管与在大鼠中报道的电生理特性相似，但我们观察到小鼠HDC神经元被动膜特性、动作电位放电和内在阈下活性膜特性有相当大的差异。总的来说，HDC神经元的电生理特性在TMN的各个亚区似乎相似，这与该核缺乏地形组织一致。此外，我们发现HDC神经元的电兴奋性没有明显的性别差异。然而，我们的数据揭示了小鼠遗传鉴定的组胺能神经元的电生理特性的多样性，这在以前的大鼠研究中没有被发现。因此，这些数据强调了小鼠遗传学的实用性，以靶定TMN内广泛存在的组胺能神经元群体，并支持了组胺能神经元是异质神经元群体的观点。
Histaminergic neurons of the tuberomammillary nucleus (TMN) are important regulators of behavioral and homeostatic processes. Previous work suggested that histaminergic neurons exhibit a characteristic electrophysiological signature, allowing for their identification in brain slice preparations. However, these previous investigations focused on neurons in the ventral subregion of the TMN of rats. Consequently, it remains unclear whether such electrophysiological properties extend to mice, including other subregions of the TMN, and the potential for differences between males and females. To further characterize the electrophysiological properties of histaminergic neurons, we performed whole-cell patch-clamp recordings on transgenic mice expressing Cre recombinase in histidine decarboxylase (HDC)-expressing cells; the sole enzyme for histamine synthesis (Hdc-cre::tdTomato). Despite similarities with the electrophysiological properties reported in rats, we observed considerable variability in mouse HDC neuron passive membrane properties, action potential firing, and intrinsic subthreshold active membrane properties. Overall, the electrophysiological properties of HDC neurons appeared similar across subregions of the TMN, consistent with a lack of topographical organization in this nucleus. Moreover, we found no obvious sex differences in the electrical excitability of HDC neurons. However, our data reveal a diversity in the electrophysiological properties of genetically identified histaminergic neurons from mice not previously appreciated from rat studies. Thus, these data highlight the utility of mouse genetics to target the widespread histaminergic neuronal population within the TMN and support the idea that histaminergic neurons are a heterogeneous neuronal population.
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