Delayed accumulation of intestinal coliform bacteria enhances life span and stress resistance in Caenorhabditis elegans fed respiratory deficient E. coli.
Delayed accumulation of intestinal coliform bacteria enhances life span and stress resistance in Caenorhabditis elegans fed respiratory deficient E. coli.
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DOI：
10.1186/1471-2180-12-300
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发表时间：
2012-12-20
期刊：
影响因子：
4.2
通讯作者：
Clarke CF
中科院分区：
文献类型：
--
作者：
Gomez F;Monsalve GC;Tse V;Saiki R;Weng E;Lee L;Srinivasan C;Frand AR;Clarke CF
线虫模型秀丽隐杆线虫的研究已经确定了保守的生物化学途径，其作用是调节寿命。寿命也会受到肠道微生物组的影响，而C。线虫的寿命会受到大肠杆菌饮食的显著影响。虽然C.线虫通常被喂食E. coli，线虫取食E.由于缺乏辅酶Q或缺乏ATP合酶而导致呼吸缺陷的大肠杆菌菌株显示出显著的寿命延长。在这里，我们探讨的机制占增强线虫寿命在这些饮食。E.通过测定作为年龄函数的蠕虫相关菌落形成单位（cfu/蠕虫或大肠菌群计数）来监测大肠杆菌。表达GFP的E.荧光显微镜下观察虫体肠内大肠杆菌的变化。蠕虫喂养标准OP 50 E。大肠杆菌菌株在L4幼虫期的肠道中具有高cfu和GFP标记的细菌，并且在成年期的第五天显示饱和的大肠菌群计数。与此相反，线虫喂养的饮食呼吸缺陷型E。缺乏辅酶Q的大肠杆菌活得更长，并且在早期不能在管腔内积累细菌。喂食复合物V缺乏细菌的动物显示出中等大肠菌群数量，寿命也不长。结果表明，呼吸缺陷型Q-少E。大肠杆菌毒素在早期成虫的咽部或肠内被有效降解，并且直到成虫的第十天才在肠道中积累。本研究结果表明，线虫喂养呼吸缺陷型大肠杆菌。大肠杆菌饮食活得更长，因为肠道细菌定殖的延迟使蠕虫比喂食OP 50 E的蠕虫承受更少的压力。大肠杆菌饮食。这项工作表明，细菌呼吸可以作为一个毒力因子，影响细菌的能力，殖民化，并随后伤害动物宿主。呼吸缺陷型细菌可能是探测高等生物肠道微生物组内益生菌关系的有用模型。
Studies with the nematode model Caenorhabditis elegans have identified conserved biochemical pathways that act to modulate life span. Life span can also be influenced by the composition of the intestinal microbiome, and C. elegans life span can be dramatically influenced by its diet of Escherichia coli. Although C. elegans is typically fed the standard OP50 strain of E. coli, nematodes fed E. coli strains rendered respiratory deficient, either due to a lack coenzyme Q or the absence of ATP synthase, show significant life span extension. Here we explore the mechanisms accounting for the enhanced nematode life span in response to these diets. The intestinal load of E. coli was monitored by determination of worm-associated colony forming units (cfu/worm or coliform counts) as a function of age. The presence of GFP-expressing E. coli in the worm intestine was also monitored by fluorescence microscopy. Worms fed the standard OP50 E. coli strain have high cfu and GFP-labeled bacteria in their guts at the L4 larval stage, and show saturated coliform counts by day five of adulthood. In contrast, nematodes fed diets of respiratory deficient E. coli lacking coenzyme Q lived significantly longer and failed to accumulate bacteria within the lumen at early ages. Animals fed bacteria deficient in complex V showed intermediate coliform numbers and were not quite as long-lived. The results indicate that respiratory deficient Q-less E. coli are effectively degraded in the early adult worm, either at the pharynx or within the intestine, and do not accumulate in the intestinal tract until day ten of adulthood. The findings of this study suggest that the nematodes fed the respiratory deficient E. coli diet live longer because the delay in bacterial colonization of the gut subjects the worms to less stress compared to worms fed the OP50 E. coli diet. This work suggests that bacterial respiration can act as a virulence factor, influencing the ability of bacteria to colonize and subsequently harm the animal host. Respiratory deficient bacteria may pose a useful model for probing probiotic relationships within the gut microbiome in higher organisms.
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影响因子：
7.4
作者：
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影响因子：
64.8
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