Variation and Diagnostic Power of the Internal Transcribed Spacer 2 in Mediterranean and Atlantic Eolid Nudibranchs (Mollusca, Gastropoda)

Variation and Diagnostic Power of the Internal Transcribed Spacer 2 in Mediterranean and Atlantic Eolid Nudibranchs (Mollusca, Gastropoda)
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地中海和大西洋裸鳃类(软体动物、腹足纲)内部转录间隔区 2 的变异和诊断能力

DOI:
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发表时间:
2021
影响因子:
3.7
通讯作者:
G. Furfaro
G. Furfaro
中科院分区:
生物学2区
文献类型:
--
作者:
M. Garzia;P. Mariottini;D. Salvi;G. Furfaro

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地中海的海洋生物多样性仍然被低估,特别是对于裸鳃类等群体。裸鳃类分类群的识别是具有挑战性的,因为很少有形态特征,其中彩色图案往往不符合物种界定。分子评估有助于揭示裸鳃类动物中隐藏的多样性,并且主要基于线粒体标记。快速进化的核标记是非常需要的,以补充物种和属水平的系统发育和系统评估。在这里,我们评估核内转录间隔区2(ITS2)的效用,以划定物种的eolid裸鳃类使用初级和二级结构。在ITS2和两个常用的线粒体标记(COI和16 S)的14个eolid类群从10个属的变化之间的比较表明ITS2检测同源,密切相关,物种的能力。虽然ITS2已在其他几种软体动物类群中卓有成效地使用,这项研究代表了第一次应用这种核标记在裸鳃类。
Mediterranean marine biodiversity is still underestimated especially for groups such as nudibranchs. The identification of nudibranchs taxa is challenging because few morphological characters are available and among them chromatic patterns often do not align with species delimitation. Molecular assessments helped unveiling cryptic diversity within nudibranchs and have been mostly based on mitochondrial markers. Fast evolving nuclear markers are much needed to complement phylogenetic and systematic assessments at the species and genus levels. Here, we assess the utility of the nuclear Internal Transcribed Spacer 2 (ITS2) to delimit species in the eolid nudibranchs using both primary and secondary structures. Comparisons between the variation observed at the ITS2 and at the two commonly used mitochondrial markers (COI and 16S) on 14 eolid taxa from 10 genera demonstrate the ability of ITS2 to detect congeneric, closely related, species. While ITS2 has been fruitfully used in several other mollusc taxa, this study represents the first application of this nuclear marker in nudibranchs.
使用4sale的RNA序列和二级结构比对的同步视觉分析和编辑。
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