Growth Factor‐Like Effects Mediated by Muscarinic Receptors in PC12M1 Cells
Growth Factor‐Like Effects Mediated by Muscarinic Receptors in PC12M1 Cells
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PC12M1 细胞中毒蕈碱受体介导的生长因子样作用
DOI：
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发表时间：
1992
影响因子：
4.7
通讯作者：
M. Sokolovsky
中科院分区：
文献类型：
--
作者：
R. Pinkas;R. Stein;L. Lindenboim;M. Sokolovsky
Abstract: Rat pheocromocytoma (PC12) cells stably expressing cloned m1 muscarinic acetylcholine receptors (PC12M1) undergo morphologic changes when stimulated by muscarinic agonists. These changes, which include the outgrowth of neurite‐like processes, are blocked by the muscarinic antagonist atropine and are not observed in PC12 cells. The observed morphological changes, which are independent of RNA and protein synthesis, are blocked by the methylation inhibitor 5′‐deoxy‐5′‐methylthioadenosine, suggesting that methylation plays a role in this process. Analysis of cyclic AMP accumulation and phosphoinositide turnover reveals that both processes are enhanced on activation by muscarinic agonist. Our data suggest, however, that the muscarinic‐dependent neurite‐like outgrowth processes are not mediated by cyclic AMP, Ca2+, or protein kinase C pathways. The muscarinic‐dependent neurite outgrowth effect is enhanced by nerve growth factor, with a resulting increase in both the number of neurite‐extending cells and the length of the neurite. In addition, activation of muscarinic receptors in PC12M1 cells stimulates the induction of marker genes for neuronal differentiation. Muscarinic receptors may therefore mediate growth factor‐like effects in these cells.
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影响因子：
2.7
作者：
KLINE, D;KLINE, JT
通讯作者：
KLINE, JT
影响因子：
3.6
作者：
F. Dörje;A. Levey;M. Brann
通讯作者：
F. Dörje;A. Levey;M. Brann
从神经递质到基因：识别缺失的环节。
影响因子：
13.8
作者：
Esterle,TM;Sanders-Bush,E
通讯作者：
Sanders-Bush,E
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DOI：
10.1152/ajpgi.1986.250.5.g691
发表时间：
1986
期刊：
The American journal of physiology
影响因子：
--
作者：
Donowitz,M;Cusolito,S;Sharp,GW
通讯作者：
Sharp,GW
大鼠脑中毒蕈碱受体刺激的磷酸肌醇代谢的发育变化。
DOI：
--
发表时间：
1987
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Balduini,W;Murphy,SD;Costa,LG
通讯作者：
Costa,LG