Pho85 kinase, a yeast cyclin‐dependent kinase, regulates the expression of UGP1 encoding UDP‐glucose pyrophosphorylase
Pho85 kinase, a yeast cyclin‐dependent kinase, regulates the expression of UGP1 encoding UDP‐glucose pyrophosphorylase
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Pho85 激酶是一种酵母细胞周期蛋白依赖性激酶，调节编码 UDP-葡萄糖焦磷酸化酶的 UGP1 的表达
作者：
M. Nishizawa;Maiko Tanabe;N. Yabuki;K. Kitada;A. Toh
PHO 85基因是酿酒酵母中PHO系统的负调节因子，编码与Cdc 28激酶（Cdc 28）高度同源的蛋白激酶（Pho 85）。已知有10种细胞周期蛋白样蛋白与Pho 85相互作用，与不同细胞周期蛋白的组合被认为负责不同的Pho 85功能，包括磷酸盐代谢，碳源利用和细胞周期调节。然而，到目前为止，仅鉴定了有限数量的Pho 85激酶的底物，包括Pho 4、Gsy 2和Sicl。为了寻找更多的Pho 85靶点，并阐明Pho 85激酶在这些细胞功能中的遗传控制机制，我们对pho 85 Δ突变对基因表达的影响进行了全基因组分析。我们发现，参与碳代谢的各种基因的表达受到突变的影响，其中，UGP 1启动子活性增加，在没有Pho 85激酶。在pho 4 Δ突变体或缺乏推定的Pho 4和Bas 2结合位点的突变体UGP 1启动子中未观察到启动子活性的这种增加，表明UGP 1表达受Pho 85通过Pho 4调节。我们还发现，碳源，生长阶段和Pho 85激酶本身改变了几个Pho 85-细胞周期蛋白基因的表达。版权所有© 2000约翰威利父子有限公司。
The PHO85 gene is a negative regulator of the PHO system in the yeast Saccharomyces cerevisiae and encodes a protein kinase (Pho85) highly homologous to the Cdc28 kinase (Cdc28). Ten cyclin‐like proteins are known to interact with Pho85, and combination with different cyclins is believed to be responsible for distinct Pho85 functions, including phosphate metabolism, carbon source utilization and cell cycle regulation. However, only a limited number of substrates of Pho85 kinase, including Pho4, Gsy2 and Sicl, have so far been identified. To search for more targets of Pho85 and to clarify the genetic control mechanisms by Pho85 kinase in these cellular functions, we carried out a genome‐wide analysis of the effect of a pho85Δ mutation on gene expression. We found that expression of various genes involved in carbon metabolism are affected by the mutation and that among them, UGP1 promoter activity was increased in the absence of Pho85 kinase. This increase in the promoter activity was not observed in a pho4Δ mutant or with a mutant UGP1 promoter that is devoid of putative Pho4 and Bas2 binding sites, suggesting that UGP1 expression is modulated by Pho85 through Pho4. We also found that expression of several Pho85–cyclin genes were altered by the carbon source, the growth phase and Pho85 kinase itself. Copyright © 2000 John Wiley & Sons, Ltd.
影响因子：
3.3
作者：
Spellman, PT;Sherlock, G;Futcher, B
通讯作者：
Futcher, B
影响因子：
56.9
作者：
DeRisi, JL;Iyer, VR;Brown, PO
通讯作者：
Brown, PO
通过抑制酿酒酵母中的细胞周期蛋白依赖性蛋白激酶，可以减轻磷酸肌醇特异性磷脂酶 C 的基本功能。
影响因子：
3.3
作者：
Flick,JS;Thorner,J
通讯作者：
Thorner,J