A multi-target lateral flow immunoassay enabling the specific and sensitive detection of total antibodies to SARS COV-2.
A multi-target lateral flow immunoassay enabling the specific and sensitive detection of total antibodies to SARS COV-2.
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DOI：
10.1016/j.talanta.2020.121737
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发表时间：
2021-02-01
期刊：
影响因子：
6.1
通讯作者：
Anfossi L
中科院分区：
文献类型：
--
作者：
Cavalera S;Colitti B;Rosati S;Ferrara G;Bertolotti L;Nogarol C;Guiotto C;Cagnazzo C;Denina M;Fagioli F;Di Nardo F;Chiarello M;Baggiani C;Anfossi L
建立了一种针对SARS冠状病毒2型(SARS CoV-2)核衣壳蛋白(N)的免疫球蛋白快速检测方法，该方法基于由两条测试线组成的多靶点横向流动免疫分析方法。这两个测试线都与几类免疫球蛋白(G、M和A)结合。通过N和金纳米颗粒形成的比色探针揭示了特异性的抗SARS免疫球蛋白。以感染总抗体反应为靶点，最早在阳性确认后7天即可达到100%的诊断特异性(95.75-100，C.I.95%，n=985健康人和其他感染者)和94.6%的敏感性(84.9-98.9，C.I.95%，n=662 SARS CoV-2感染者)。除了快速检测的早期检测能力外，获得了与参考血清学ELISA法一致的结果。对三名血清转换患者的随访证实了不同免疫球蛋白随机上升的假设，并加强了“总抗体”方法，以可靠地检测对SARS CoV-2感染的血清学反应。建立了一种基于侧向流动免疫分析技术的快速检测SARS CoV-2感染的总血清学应答的方法。检测新冠肺炎抗体的一种灵敏、特异的侧向流动免疫分析方法捕获总免疫球蛋白的广谱特效剂能够提高灵敏度。两个测试线被组合在一起，以保证没有假阳性结果。基于多靶点LFIA的诊断可以作为分子检测的补充。
A rapid test for detecting total immunoglobulins directed towards the nucleocapsid protein (N) of severe acute syndrome coronavirus 2 (SARS CoV-2) was developed, based on a multi-target lateral flow immunoassay comprising two test lines. Both test lines bound to several classes of immunoglobulins (G, M, and A). Specific anti-SARS immunoglobulins were revealed by a colorimetric probe formed by N and gold nanoparticles. Targeting the total antibodies response to infection enabled achieving 100% diagnostic specificity (95.75–100, C.I. 95%, n = 85 healthy and with other infections individuals) and 94.6% sensitivity (84.9–98.9, C.I. 95%, n = 62 SARS CoV-2 infected subjects) as early as 7 days post confirmation of positivity. Agreeing results with a reference serological ELISA were achieved, except for the earlier detection capability of the rapid test. Follow up of the three seroconverting patients endorsed the hypothesis of the random rise of the different immunoglobulins and strengthened the ‘total antibodies’ approach for the trustworthy detection of serological response to SARS CoV-2 infection. A rapid test based on the lateral flow immunoassay technology was established to detect the total serological response to the SARS CoV-2 infection. A sensitive and specific lateral flow immunoassay for detecting antibodies to Covid-19. Broad-specific agents to capture total immunoglobulins enabled increasing the sensitivity. Two test lines were combined to guarantee no false positive results. Diagnosis based on the multi-target LFIA can complement molecular assays.
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