Alterations in the Salivary Proteome and N-Glycome of Sjögren's Syndrome Patients.
Alterations in the Salivary Proteome and N-Glycome of Sjögren's Syndrome Patients.
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DOI：
10.1021/acs.jproteome.6b01051
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发表时间：
2017-04-07
影响因子：
4.4
通讯作者：
Fisher, Susan J.
中科院分区：
文献类型：
--
作者：
Hall, Steven C.;Hassis, Maria E.;Williams, Katherine E.;Albertolle, Matthew E.;Prakobphol, Akrapom;Dykstra, Andrew B.;Laurance, Megan;Ona, Katherine;Niles, Richard K.;Prasad, Namrata;Gormley, Matthew;Shiboski, Caroline;Criswell, Lindsey A.;Witkowska, H. Ewa;Fisher, Susan J.
关键词：
我们使用基于同量异位素质量标记（iTRAQ）和凝集素亲和捕获质谱（MS）的工作流程，对从诊断为原发性干燥综合征（pSS）的患者中获得的腮腺唾液（PS）和全唾液（WS）样本进行了全球分析，这些患者入选了干燥综合征国际合作临床联盟（SICCA），并与两个对照组进行了比较。iTRAQ分析揭示了可能参与疾病过程的许多蛋白质的上调和下调（例如，组蛋白）或试图减轻随后的损害（例如，杀菌/渗透性增加折叠包含家族（BPIF）成员）。应用于独立样本集的免疫印迹方法证实了pSS相关的β2-微球蛋白（PS中）上调以及碳酸酐酶VI（WS中）和BPIFB 2（PS中）下调。除了蛋白质组，我们分析了pSS和对照样品的N-糖基。它们使用凝集素Aleuria aurantia和麦胚凝集素富集糖肽，这两种凝集素分别识别岩藻糖和唾液酸/N-乙酰葡糖胺。MS分析表明，pSS与PS和WS中许多唾液糖蛋白的N-糖基化增加有关。唾液蛋白质组和N-糖组的观察到的改变可用作pSS生物标志物，使更容易和更早地检测这种综合征，同时提供潜在的疾病过程的新见解。
We used isobaric mass tagging (iTRAQ) and lectin affinity capture mass spectrometry (MS)-based workflows for global analyses of parotid saliva (PS) and whole saliva (WS) samples obtained from patients diagnosed with primary Sjögren’s Syndrome (pSS) who were enrolled in the Sjögren’s International Collaborative Clinical Alliance (SICCA) as compared with two control groups. The iTRAQ analyses revealed up- and down-regulation of numerous proteins that could be involved in the disease process (e.g., histones) or attempts to mitigate the ensuing damage (e.g., bactericidal/permeability increasing fold containing family (BPIF) members). An immunoblot approach applied to independent sample sets confirmed the pSS associated up-regulation of β2-microglobulin (in PS) and down-regulation of carbonic anhydrase VI (in WS) and BPIFB2 (in PS). Beyond the proteome, we profiled the N-glycosites of pSS and control samples. They were enriched for glycopeptides using lectins Aleuria aurantia and wheat germ agglutinin, which recognize fucose and sialic acid/N-acetyl glucosamine, respectively. MS analyses showed that pSS is associated with increased N-glycosylation of numerous salivary glycoproteins in PS and WS. The observed alterations of the salivary proteome and N-glycome could be used as pSS biomarkers enabling easier and earlier detection of this syndrome while lending potential new insights into the disease process.
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影响因子：
7
作者：
Chaudhury, Nayab M. A.;Proctor, Gordon B.;Flowers, Sarah A.
通讯作者：
Flowers, Sarah A.
影响因子：
3.8
作者：
Derry, Man E. C.;Sutherland, Michael R.;Pryzdial, Edward L. G.
通讯作者：
Pryzdial, Edward L. G.
影响因子：
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作者：
Daniels, Troy E.;Criswell, Lindsey A.;Shiboski, Caroline;Shiboski, Stephen;Lanfranchi, Hector;Dong, Yi;Schiodt, Morten;Umehara, Hisanori;Sugai, Susumu;Challacombe, Stephen;Greenspan, John S.
通讯作者：
Greenspan, John S.
影响因子：
3.4
作者：
Ambatipudi, Kiran S.;Swatkoski, Stephen;Moresco, James J.;Tu, Patricia G.;Coca, Andreea;Anolik, Jennifer H.;Gucek, Marjan;Sanz, Ignacio;Yates, John R., III;Melvin, James E.
通讯作者：
Melvin, James E.
DOI：
10.1016/j.gpb.2015.06.002
发表时间：
2015-08
期刊：
Genomics, proteomics & bioinformatics
影响因子：
--
作者：
Chen W;Cao H;Lin J;Olsen N;Zheng SG
通讯作者：
Zheng SG