The effect of the TLR9 ligand CpG-oligodeoxynucleotide on the protective immune response to alcelaphine herpesvirus-1-mediated malignant catarrhal fever in cattle.
The effect of the TLR9 ligand CpG-oligodeoxynucleotide on the protective immune response to alcelaphine herpesvirus-1-mediated malignant catarrhal fever in cattle.
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DOI：
10.1186/1297-9716-45-59
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发表时间：
2014-05-27
影响因子：
4.4
通讯作者：
Haig DM
中科院分区：
文献类型：
--
作者：
Parameswaran N;Russell GC;Bartley K;Grant DM;Deane D;Todd H;Dagleish MP;Haig DM
我们希望确定 CpG ODN 佐剂对阿尔塞拉芬疱疹病毒 1 (AlHV-1) 恶性卡他热 (MCF)（一种牛的致命性淋巴组织增生性疾病）的保护性免疫的强度和持续时间的影响。免疫与病毒中和抗体的粘膜屏障有关。结果表明，CpG ODN 与乳剂佐剂和减毒 AlHV-1 (atAlHV-1) 一起或单独与 atAlHV-1 一起使用，不会影响对临床疾病的总体保护或使用乳剂和 atAlHV-1 实现的免疫持续时间。这与在使用 BoHV-1 的牛或使用各种其他免疫原的牛和猪中进行的其他类似研究形成鲜明对比。除此之外，还进行了其他一些先前未报道过的新颖观察。首先，我们能够统计证实针对 MCF 的疫苗保护与鼻腔分泌物中的病毒中和抗体 (nAb) 相关，但与血浆中的抗体无关，也不与鼻腔分泌物或血浆中的总病毒特异性抗体 (tAb) 滴度相关。此外，CpG ODN 单独作为佐剂不支持病毒中和抗体的产生。其次，比较攻击前后的滴度，MCF 动物的 tAb 显着增加。在受保护的动物中没有发现这种情况。最后，用乳剂和 atAlHV-1 免疫的动物中存在强烈的 IFN-γ 反应，如通过培养物中分泌 IFN-γ 的 PBMC（且缺乏 IL-4）所测量的，该反应不受包含 CpG ODN 的影响。这表明口鼻咽区域的 nAb 对于预防 AlHV-1 MCF 很重要。
We wished to determine the effect of of CpG ODN adjuvant on the magnitude and duration of protective immunity against alcelaphine herpesvirus-1 (AlHV-1) malignant catarrhal fever (MCF), a fatal lymphoproliferative disease of cattle. Immunity was associated with a mucosal barrier of virus-neutralising antibody. The results showed that CpG ODN included either with emulsigen adjuvant and attenuated AlHV-1 (atAlHV-1) or alone with atAlHV-1 did not affect the overall protection from clinical disease or duration of immunity achieved using emulsigen and atAlHV-1. This is in contrast to other similar studies in cattle with BoHV-1 or cattle and pigs with various other immunogens. In addition to this, several other novel observations were made, not reported previously. Firstly, we were able to statistically verify that vaccine protection against MCF was associated with virus-neutralising antibodies (nAbs) in nasal secretions but was not associated with antibodies in blood plasma, nor with total virus-specific antibody (tAb) titres in either nasal secretions or blood plasma. Furthermore, CpG ODN alone as adjuvant did not support the generation of virus-neutralising antibodies. Secondly, there was a significant boost in tAb in animals with MCF comparing titres before and after challenge. This was not seen with protected animals. Finally, there was a strong IFN-γ response in animals with emulsigen and atAlHV-1 immunisation, as measured by IFN-γ secreting PBMC in culture (and a lack of IL-4) that was not affected by the inclusion of CpG ODN. This suggests that nAbs at the oro-nasal-pharyngeal region are important in protection against AlHV-1 MCF.
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影响因子：
5.5
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通讯作者：
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作者：
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通讯作者：
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影响因子：
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作者：
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