Investigation of the effects of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism method.
Investigation of the effects of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism method.
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DOI：
10.1021/la8036814
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发表时间：
2009-03-03
期刊：
影响因子：
--
通讯作者：
Latour RA
中科院分区：
文献类型：
--
作者：
Sivaraman B;Fears KP;Latour RA
在本文中，我们提出了一种使用圆形二色光谱偏振法的方法的发展，这种方法使用定制设计的比皿来增加测量吸附蛋白质二级结构的信噪比，从而提高了灵敏度和再现性。然后应用这些方法来研究表面化学和溶液浓度如何影响吸附蛋白质的数量及其二级结构。用稀释溶液（0.1 mg/mL）和中等浓度溶液（1.0 mg/mL）中的CH3、OCH2-CF3、NH2、COOH和OH末端基团将人纤维蛋白原和白蛋白吸附在金上的烷硫醇自组装单层（SAMs）上。研究发现，表面疏水性的增加会导致蛋白质吸附量的增加和由吸附过程引起的结构变化程度的增加，而溶液浓度的增加会导致蛋白质吸附量的增加，但构象变化程度的降低，这些影响在疏水性越强的表面上更为明显。结合使用这两个参数（即表面化学和溶液浓度）提供了一种方法，可以从吸附蛋白质的量独立地改变吸附后结构变化的程度。进一步的研究正在进行中，以检查这些因素中哪一个对血小板反应影响最大，其总体目标是更好地了解控制生物材料表面血液相容性的基本因素。
In this paper we present the development of methods using circular dichroism spectropolarimetry with a custom-designed cuvette to increase the signal-to-noise ratio for the measurement of the secondary structure of adsorbed proteins, thus providing enhanced sensitivity and reproducibility. These methods were then applied to investigate how surface chemistry and solution concentration influence both the amount of adsorbed proteins and their secondary structure. Human fibrinogen and albumin were adsorbed onto alkanethiol self-assembled monolayers (SAMs) on gold with CH3, OCH2-CF3, NH2, COOH, and OH terminal groups from both dilute (0.1 mg/mL) and moderately concentrated (1.0 mg/mL) solutions. An increase in surface hydrophobicity was found to cause an increase in both the amount of the protein adsorbed and the degree of structural change that was caused by the adsorption process, while an increase in solution concentration caused an increase in the amount of protein adsorbed but a decrease in the degree of conformational change, with these effects being more pronounced on the more hydrophobic surfaces. The combined use of these two parameters (i.e., surface chemistry and solution concentration) thus provides a means of independently varying the degree of structural change following adsorption from the amount of adsorbed protein. Further studies are underway to examine which of these factors most strongly influences platelet response, with the overall goal of developing a better understanding of the fundamental factors governing the hemocompatibility of biomaterial surfaces.
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影响因子：
3.9
作者：
Fears, Kenan P.;Creager, Stephen E.;Latour, Robert A.
通讯作者：
Latour, Robert A.
影响因子：
14
作者：
Chittur, KK
通讯作者：
Chittur, KK
影响因子：
4.9
作者：
Latour, Robert A.
通讯作者：
Latour, Robert A.
DOI：
10.1016/0005-2795(71)90282-0
发表时间：
1971-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
BUDZYNSKI, AZ
通讯作者：
BUDZYNSKI, AZ
影响因子：
2.9
作者：
CASSIM, JY;YANG, JT
通讯作者：
YANG, JT