BMP signaling controls formation of a primordial germ cell niche within the early genital ridges.
BMP signaling controls formation of a primordial germ cell niche within the early genital ridges.
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DOI：
10.1016/j.ydbio.2010.04.011
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发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Molyneaux K
中科院分区：
文献类型：
--
作者：
Dudley B;Palumbo C;Nalepka J;Molyneaux K
干细胞对于维持组织稳态是必要的，而这些细胞周围的微环境（又称生态位）控制着它们自我更新或分化的能力。对于许多干细胞群来说，尚不清楚哪些细胞和信号构成了一个生态位。在这里，我们确定了小鼠生殖脊中可能的 PGC 生态位。 Bmpr1a 的条件性消融被用来证明 BMP 信号传导对于 PGC 的存活和迁移是必需的，因为这些细胞定植于生殖嵴。生殖脊内 BMP 信号传导减少导致中肾间充质内体细胞死亡增加。这些支持细胞的损失与中肾标志物 Pax2 水平的降低以及体腔上皮中表达的基因（包括假定的 PGC 化学引诱剂 Kit1 和 Sdf1a）的减少相关。我们认为 BMP 信号传导促进生殖嵴内的中肾细胞存活，并且这些细胞支持体腔上皮（PGC 迁移的目标）的正确发育。 BMP 信号丢失会导致 PGC 靶点丢失，从而导致 PGC 数量减少并扰乱 PGC 迁移。
Stem cells are necessary to maintain tissue homeostasis and the microenvironment (a.k.a. the niche) surrounding these cells controls their ability to self renew or differentiate. For many stem cell populations it remains unclear precisely what cells and signals comprise a niche. Here we identify a possible PGC niche in the mouse genital ridges. Conditional ablation of Bmpr1a was used to demonstrate that BMP signaling is required for PGC survival and migration as these cells colonize the genital ridges. Reduced BMP signaling within the genital ridges led to increased somatic cell death within the mesonephric mesenchyme. Loss of these supporting cells correlated with decreased levels of the mesonephric marker, Pax2, as well as a reduction in genes expressed in the coelomic epithelium including the putative PGC chemo-attractants Kitl and Sdf1a. We propose that BMP signaling promotes mesonephric cell survival within the genital ridges and that these cells support correct development of the coelomic epithelium, the target of PGC migration. Loss of BMP signaling leads to the loss of the PGC target resulting in reduced PGC numbers and disrupted PGC migration.
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