Human cytotoxic T cell structures associated with expression of cytolysis. I. Analysis at the clonal cell level of the cytolysis‐inhibiting effect of 7 monoclonal antibodies
Human cytotoxic T cell structures associated with expression of cytolysis. I. Analysis at the clonal cell level of the cytolysis‐inhibiting effect of 7 monoclonal antibodies
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与细胞溶解表达相关的人细胞毒性 T 细胞结构 I. 7 种单克隆抗体细胞溶解抑制作用的克隆细胞水平分析。
DOI：
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发表时间：
1982
影响因子：
5.4
通讯作者：
C. Mawas
中科院分区：
文献类型：
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作者：
B. Malissen;N. Rebai;A. Liabeuf;C. Mawas
筛选用人抗HLA-A2克隆的细胞毒性T淋巴细胞（CTL）免疫的BALB/c小鼠衍生的单克隆抗体（mAb）在不存在补体的情况下阻断免疫CTL克隆的细胞溶解活性的能力。已衍生出8种细胞溶解抑制mAb。其中一种针对HLA I类分子上表达的单态决定簇，因此可能通过靶细胞抗原掩蔽效应抑制细胞溶解。其他7种mAb识别“CTL功能相关结构”，并且不必干扰靶细胞抗原以抑制细胞溶解。这7株mAb的F（ab '）2和Fab片段也具有抑制作用。结合的竞争性抑制和初步生物化学分析表明，当不进行还原分析时，这7种mAb在克隆的CTL上定义了30 kDa二硫键结合成几种多聚体形式的结构的各种表位。已对一系列短期（1个月）和长期（> 10个月）扩增克隆CTL系进行了研究，这些克隆CTL系体外来源于表现出各种特异性的体内同种异体致敏个体。出乎意料的是，这些CTL克隆中只有10%被抑制。流式细胞荧光分析进一步显示，非抑制性和抑制性CTL克隆表达相似量的30 kDa结构。因此，当在克隆水平上分析时，CTL的抑制是异质性的，而不仅仅与这种结构的存在或不存在有关。此外，CTL克隆被抑制的能力似乎与其HLA-A、B或C特异性或其裂解活性无关。这种mAb定义的结构和CTL功能之间的联系进行了讨论。
Monoclonal antibodies (mAb) derived from BALB/c mice immunized with human anti‐HLA‐A2 cloned cytotoxic T lymphocytes (CTL) were screened for their ability to block, in the absence of complement, the cytolytic activity of the immunizing CTL clone. Eight cytolysis‐inhibiting mAb have been derived. One of these was directed against a monomorphic determinant expressed on HLA‐class I molecules and thus probably inhibited cytolysis via a target cell antigen‐masking effect. The 7 other mAb recognized “CTL function‐associated structures” and did not have to interfere with target cell antigens in order to inhibit cytolysis. F(ab')2 and Fab fragments of these 7 mAb were also inhibitory. Competitive inhibition of binding and preliminary biochemical analysis suggested that these 7 mAb defined on cloned CTL various epitopes of a structure of 30 kDa disulfide‐bonded into several multimeric forms when analyzed without reduction. The inhibitory effect of these 7 mAb has been investigated on a series of short‐term (1 month) and long‐term (> 10 months) expanded cloned CTL lines derived in vitro from an in vivo allosensitized individual exhibiting various specificities. Unexpectedly, only 10% of these CTL clones were inhibited. Flow cytofluorimetric analysis further revealed that noninhibited and inhibited CTL clones expressed similar amounts of the 30‐kDa structure. Consequently, the inhibition of CTL was heterogeneous when analyzed at the clonal level and not simply related to the presence or absence of this structure. Furthermore, the ability of CTL clones to be inhibited appeared to be unrelated to their HLA‐A, B or C specificity or to their lytic activity. The connections between this mAb‐defined structure and CTL function are discussed.
影响因子：
4.4
作者：
M. Sarmiento;A. Glasebrook;F. Fitch
通讯作者：
M. Sarmiento;A. Glasebrook;F. Fitch
影响因子：
4.4
作者：
Konrad Kurzinger;Timothy Reynolds;Ronald N. Germain;D. Davignon;Eric Martz;T. A. Springer
通讯作者：
Konrad Kurzinger;Timothy Reynolds;Ronald N. Germain;D. Davignon;Eric Martz;T. A. Springer
DOI：
10.1073/pnas.78.7.4535
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
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作者：
DAVIGNON, D;MARTZ, E;SPRINGER, TA
通讯作者：
SPRINGER, TA