Muramyl dipeptide enhances lipopolysaccharide-induced osteoclast formation and bone resorption through increased RANKL expression in stromal cells.
Muramyl dipeptide enhances lipopolysaccharide-induced osteoclast formation and bone resorption through increased RANKL expression in stromal cells.
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Muramyl二肽增强了脂多糖诱导的破骨细胞形成和骨吸收，通过增加的基质细胞中的RANKL表达。
DOI：
10.1155/2015/132765
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发表时间：
2015
影响因子：
4.1
通讯作者：
Takano-Yamamoto T
中科院分区：
文献类型：
--
作者：
Ishida M;Kitaura H;Kimura K;Sugisawa H;Aonuma T;Takada H;Takano-Yamamoto T
脂多糖是一种细菌细胞壁成分，可诱导破骨细胞形成和病理性骨吸收。胞壁酰二肽(MDP)是负责肽聚糖免疫活性的最小基本结构单位，在细菌中广泛表达。在本研究中，我们研究了MDP在脂多糖诱导的破骨细胞形成和骨吸收中的作用。将含或不含MDP的脂多糖分别注入小鼠颅骨上区。MDP组和LPS组小鼠破骨细胞数、组织蛋白酶K和抗酒石酸酸性磷酸酶(TRAP)基因表达水平、骨破坏面积比、抗酒石酸酸性磷酸酶5b(TRACP 5b)和I型胶原C端末端肽片段数均高于单独用药组。另一方面，MDP对甲状旁腺激素诱导的小鼠破骨细胞生成无明显影响。亚甲基二磷酸在体内和体外均能增强脂多糖诱导的核因子κB受体激活剂和Toll样受体4的表达。MDP还增强了脂多糖诱导的基质细胞中的丝裂原活化蛋白激酶(MAPK)信号，包括ERK、p38和JNK。提示MDP在细菌感染性疾病的病理性骨吸收中可能起重要作用。
Lipopolysaccharide (LPS) is bacterial cell wall component capable of inducing osteoclast formation and pathological bone resorption. Muramyl dipeptide (MDP), the minimal essential structural unit responsible for the immunological activity of peptidoglycans, is ubiquitously expressed by bacterium. In this study, we investigated the effect of MDP in LPS-induced osteoclast formation and bone resorption. LPS was administered with or without MDP into the supracalvariae of mice. The number of osteoclasts, the level of mRNA for cathepsin K and tartrate-resistant acid phosphatase (TRAP), the ratio of the bone destruction area, the level of tartrate-resistant acid phosphatase form 5b (TRACP 5b), and C-terminal telopeptides fragments of type I collagen as a marker of bone resorption in mice administrated both LPS and MDP were higher than those in mice administrated LPS or MDP alone. On the other hand, MDP had no effect on osteoclastogenesis in parathyroid hormone administrated mice. MDP enhanced LPS-induced receptor activator of NF-κB ligand (RANKL) expression and Toll-like receptor 4 (TLR4) expression in vivo and in stromal cells in vitro. MDP also enhanced LPS-induced mitogen-activated protein kinase (MAPK) signaling, including ERK, p38, and JNK, in stromal cells. These results suggest that MDP might play an important role in pathological bone resorption in bacterial infection diseases.
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影响因子：
4.4
作者：
Kitaura, H;Sands, MS;Teitelbaum, SL
通讯作者：
Teitelbaum, SL
影响因子：
--
作者：
ORCEL, P;FEUGA, M;DEVERNEJOUL, MC
通讯作者：
DEVERNEJOUL, MC
影响因子：
3.5
作者：
Chung, Y. -H.;Chang, E. -J.;Ko, J. S.
通讯作者：
Ko, J. S.
影响因子：
6
作者：
Merkel, KD;Erdmann, JM;Teitelbaum, SL
通讯作者：
Teitelbaum, SL
影响因子：
4.8
作者：
Chow, JC;Young, DW;Gusovsky, F
通讯作者：
Gusovsky, F