Imprinting analysis of porcine MAGEL2 gene in two fetal stages and association analysis with carcass traits
Imprinting analysis of porcine MAGEL2 gene in two fetal stages and association analysis with carcass traits
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猪MAGEL2基因在胎儿两期的印记分析及与胴体性状的关联分析
DOI：
10.1007/s11033-011-0719-0
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发表时间：
2011
影响因子：
2.8
通讯作者：
Deng, Chang-Yan
中科院分区：
文献类型：
--
作者：
Wang, Chao;Zheng, Rong;Xiong, Yuan-Zhu;Deng, Chang-Yan
印迹基因在胚胎生长发育和胎盘功能的调控中起着重要作用，但目前在猪中研究的印迹基因数量有限。在这项研究中，我们克隆和鉴定猪MAGEL 2（黑色素瘤抗原样基因2），并确定其在猪胎儿发育过程中的印记状态。分离出编码1，193个氨基酸的完整开放阅读框（ORF），并在编码区鉴定出2个单核苷酸多态性（SNP）（g.2592 A C和g.3277 T C）。采用约克郡×梅山F1正反交模型，采用RT-PCR/RFLP方法检测了猪MAGEL 2基因在妊娠30 d和65 d两个发育阶段的印迹状态。印迹分析表明，猪MAGEL 2在65天的胎儿组织，包括心脏，肝脏，脾脏，肺，肾脏，胃，小肠，骨骼肌，脑和胎盘中呈父系表达。有趣的是，我们在约克郡公猪×梅山母猪杂交产生的30个dpc胎儿中观察到MAGEL 2基因的印记变异，其中7个杂合子胎儿来自父本等位基因的单等位基因表达，2个来自父本和母本等位基因的双等位基因表达。对约克郡×梅山F2资源群体的关联分析表明，g.2592 A C突变与屠宰率（P0.05）和背膘厚（P0.05）显著相关。研究结果表明，MAGEL 2作为一个新的猪印记基因，可能是影响胴体性状的候选基因，为印记基因在猪发育过程中的功能研究提供了重要信息。
Imprinted genes play an essential role in the regulation of fetal growth, development and function of the placenta, however only a limited number of imprinted genes have been studied in swine. In this study, we cloned and characterized porcine MAGEL2 (melanoma antigen-like gene 2), and also identified its imprinting status during porcine fetal development. The complete open reading frame (ORF) encoding 1,193 amino acids was isolated and two single nucleotide polymorphisms (SNPs) (g.2592A>C and g.3277T>C) in the coding region were identified. The reciprocal Yorkshire × Meishan F1 hybrid model and the RT-PCR/RFLP method were used to detect the imprinting status of porcine MAGEL2 gene at two developmental stages of day 30 and 65 of gestation. Imprinting analysis showed that porcine MAGEL2 was paternally expressed in day 65 fetal tissues, including heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, brain and placenta. Interestingly, we observed an imprinting variance of MAGEL2 gene in 30 dpc fetuses produced by the cross of Yorkshire boar × Meishan sow, in which seven heterozygous fetuses were monoallelically expressed from the paternal allele but two were biallelically expressed from both the paternal and maternal alleles. Association analysis in a Yorkshire × Meishan F2 resource population showed that the mutation of g.2592A>C was significantly associated with dressed carcass percentage (P < 0.05) and buttock fat thickness (P < 0.05). Our results suggest that MAGEL2, as a novel imprinted gene in pig, might be a candidate gene affecting carcass traits and could provide important information for the functional study of imprinted genes during porcine development.
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影响因子：
9.8
作者：
Wolf JB;Hager R
通讯作者：
Hager R
影响因子：
3.3
作者：
J. Estellé;A. Mercadé;J. Noguera;M. Pérez-Enciso;C. Óvilo;A. Sánchez;J. Folch
通讯作者：
J. Estellé;A. Mercadé;J. Noguera;M. Pérez-Enciso;C. Óvilo;A. Sánchez;J. Folch
影响因子：
3.5
作者：
Lee, S;Walker, CL;Wevrick, R
通讯作者：
Wevrick, R
影响因子：
3.8
作者：
A. Thurston;Jane E. Taylor;J. Gardner;K. Sinclair;L. Young
通讯作者：
A. Thurston;Jane E. Taylor;J. Gardner;K. Sinclair;L. Young
影响因子：
3.5
作者：
Bischof, Jocelyn M.;Stewart, Colin L.;Wevrick, Rachel
通讯作者：
Wevrick, Rachel