Neurite outgrowth from mammalian CNS neurons on astrocytesin vitro may not be mediated primarily by laminin
Neurite outgrowth from mammalian CNS neurons on astrocytesin vitro may not be mediated primarily by laminin
复制标题
体外星形胶质细胞上哺乳动物中枢神经系统神经元的神经突生长可能主要不是由层粘连蛋白介导的
DOI：
10.1007/bf01735385
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
S. David
中科院分区：
文献类型：
--
作者：
S. David
许多体外研究表明，细胞外基质（ECM）的某些成分，如糖蛋白层粘连蛋白，可以促进神经突的生长。在本研究中，研究了层粘连蛋白和硫酸肝素蛋白多糖（另一种ECM分子）在体外中枢神经胶质细胞（星形胶质细胞）和非胶质细胞（轻脑膜）细胞上的存在。此外，这些细胞和层粘连蛋白包被的组织培养基质促进发育中的哺乳动物小脑皮质神经元的神经突生长的能力也被检测。研究发现，与星形胶质细胞相比，瘦脑膜细胞被层粘连蛋白和硫酸肝素蛋白多糖抗体所标记的程度要高得多。然而，星形胶质细胞单层上延伸神经突的神经元比例是轻脑膜上的4倍，是层粘连蛋白上的2倍。此外，星形胶质细胞单层上生长的神经突的长度比层粘连蛋白或轻脑膜长3 - 4倍。在星形胶质细胞条件培养基中培养并不能促进轻脑膜和层粘连蛋白上的神经突生长。我们还研究了各种抗体在这些单层膜上阻断神经突生长的能力。这些结果表明，哺乳动物中枢神经系统神经元在星形胶质细胞上的生长可能是通过星形胶质细胞表面不同于层粘连蛋白的分子介导的。
SummaryA number ofin vitro studies suggest that certain components of the extracellular matrix (ECM), such as the glycoprotein laminin, can promote neurite outgrowth. In the present study the presence of laminin and heparan sulphate proteoglycan, another ECM molecule, on CNS glial (astrocytes) and non-glial (leptomeningeal) cellsin vitro was examined. In addition, the ability of these cells and laminin-coated tissue culture substrates to promote neurite outgrowth from developing mammalian cerebellar cortical neurons was also assayed. Leptomeningeal cells were found to be labelled much more intensely with antibodies against laminin and heparin sulphate proteoglycan than were astrocytes. However, the proportion of neurons extending neurites was fourfold greater on astrocyte monolayers than on leptomeninges, and twofold greater than that on laminin. In addition, the length of the neurites growing on astrocyte monolayers was three- to fourfold greater than that on laminin or leptomeninges. Neurite outgrowth on leptomeninges and laminin could not be enhanced by culturing in astrocyte conditioned medium. The ability of various antibodies to block neurite outgrowth on these monolayers was also studied. These results suggest that the robust neurite outgrowth from mammalian CNS neurons plated on astrocytes may be mediated via an astrocyte surface molecule distinct from laminin.
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影响因子：
2.7
作者：
ROGERS, SL;LETOURNEAU, PC;FURCHT, LT
通讯作者：
FURCHT, LT
新的神经元生长因子。
影响因子：
13.9
作者：
Berg,DK
通讯作者：
Berg,DK
神经系统诱导、组织发生和围产期发育过程中细胞粘附的调节。
影响因子：
13.9
作者：
Edelman,GM
通讯作者：
Edelman,GM
HNK-1 (Leu-7) 人淋巴细胞的表征。
DOI：
--
发表时间：
1982
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Abo,T;Balch,CM
通讯作者：
Balch,CM
DOI：
10.1073/pnas.82.7.2183
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANDER, AD;FUJII, DK;REICHARDT, LF
通讯作者：
REICHARDT, LF