Purple Potato Extract Promotes Intestinal Epithelial Differentiation and Barrier Function by Activating AMP-Activated Protein Kinase.
Purple Potato Extract Promotes Intestinal Epithelial Differentiation and Barrier Function by Activating AMP-Activated Protein Kinase.
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DOI：
10.1002/mnfr.201700536
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发表时间：
2018-03
影响因子：
5.2
通讯作者：
Zhu MJ
中科院分区：
文献类型：
--
作者：
Sun X;Du M;Navarre DA;Zhu MJ
肠道上皮屏障功能的紊乱会引起炎症和其他源于肠道的健康问题。紫薯含有大量有益的多酚类化合物。本研究旨在通过Caco-2细胞和离体培养肠道组织研究紫薯提取物（PPE）对肠道分化和屏障功能的影响，并探讨其作用机制。PPE增加了Caco-2细胞的上皮电阻值，降低了Caco-2细胞的fitc -葡聚糖细胞旁通量，这与Caco-2细胞和离体肠道的肠上皮分化增强有关。此外，PPE处理增强了amp活化蛋白激酶（AMPK）的活性，同时增加了CDX2的表达，CDX2是调节肠上皮分化的关键转录因子。利用CRISPR/Cas9系统敲除AMPK， PPE对肠上皮分化和屏障功能的积极作用消失，同时CDX2的表达降低。PPE通过激活AMPK改善肠道上皮分化和屏障功能，这表明PPE以及相关的紫薯食用可以作为改善肠道上皮健康的一种支持性饮食治疗策略。
Perturbation of gut epithelial barrier function induces inflammation and other health problems that originate from the gut. Purple potato contains a high content of beneficial polyphenolic compounds. The objective of this study was to evaluate the effect of purple potato extract (PPE) on intestinal differentiation and barrier function, and explore its underlying mechanism using Caco-2 cells and ex vivo cultured gut tissues. PPE increased transepithelial electrical resistance and decreased FITC-dextran paracellular flux in Caco-2 cells, which were associated with strengthened intestinal epithelial differentiation in both Caco-2 cells and ex vivo guts. Furthermore, PPE treatment enhanced AMP-activated Protein Kinase (AMPK) activity, concomitant with the increased expression of CDX2, a key transcriptional factor regulating intestinal epithelial differentiation. Knocking out AMPK using CRISPR/Cas9 system abolished the positive effects of PPE on intestinal epithelial differentiation and barrier function, in junction with the reduced expression of CDX2. PPE improved gut epithelial differentiation and barrier function via activating AMPK, indicating that PPE, as well as associated purple potato consumption, could be used as a supportive dietary therapeutic strategy for improving gut epithelial health.
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影响因子：
2.4
作者：
Bischoff SC;Barbara G;Buurman W;Ockhuizen T;Schulzke JD;Serino M;Tilg H;Watson A;Wells JM
通讯作者：
Wells JM
影响因子：
14.2
作者：
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通讯作者：
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影响因子：
4.2
作者：
Amasheh, Maren;Schlichter, Susanne;Schulzke, Joerg D.
通讯作者：
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影响因子：
5.5
作者：
Novak EA;Mollen KP
通讯作者：
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影响因子：
3.7
作者：
Ong KW;Hsu A;Tan BK
通讯作者：
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