Effect of Resistance Training on Extracellular Matrix Adaptations in Skeletal Muscle of Older Rats.
Effect of Resistance Training on Extracellular Matrix Adaptations in Skeletal Muscle of Older Rats.
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DOI：
10.3389/fphys.2018.00374
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发表时间：
2018
影响因子：
4
通讯作者：
Durigan JLQ
中科院分区：
文献类型：
--
作者：
Guzzoni V;Ribeiro MBT;Lopes GN;de Cássia Marqueti R;de Andrade RV;Selistre-de-Araujo HS;Durigan JLQ
随着年龄的增长，在骨骼肌中观察到结缔组织，特别是细胞外基质（ECM）蛋白的积累。阻力训练（RT）已被广泛推荐用于减轻年龄诱导的肌减少症，即使其对控制骨骼肌中ECM周转的组分的影响仍有待阐明。因此，本研究的目的是确定RT对老年大鼠骨骼肌中ECM关键成分的结缔组织含量和基因表达的影响。年轻（3个月）年龄较大（21个月）成年雄性Wistar大鼠接受RT方案（65、85、95和100%负荷的爬梯），每周3次，持续12周。训练后48小时，解剖比目鱼肌（SOL）和腓肠肌（GAS）肌肉进行组织学和mRNA分析。RT缓解了两种肌肉中与年龄相关的结缔组织含量的增加，即使在老年训练大鼠中COL-1和COL-3的mRNA水平升高。总体而言，RT显著提高了老年大鼠GAS和SOL肌肉中结缔组织沉积关键成分（TGFβ和CTGF; MMP-2和-9; TIMP-1和-2）的基因表达。总之，RT减弱了年龄诱导的结缔组织积累，伴随着与老年大鼠SOL和GAS肌肉中ECM网络的合成和降解相关的基因的上调。虽然我们的研究结果表明，RT起着至关重要的作用，减少老年后肢肌肉结缔组织的积累，ECM营业额的关键组成部分矛盾地升高。RT诱导的表型反应并不伴随着ECM周转相关成分的基因表达。
Accumulation of connective tissue, particularly extracellular matrix (ECM) proteins, has been observed in skeletal muscles with advancing age. Resistance training (RT) has been widely recommended to attenuate age-induced sarcopenia, even though its effects on the components that control ECM turnover in skeletal muscles remain to be elucidated. Thus, the aim of this study was to determine the effects of RT on connective tissue content and gene expression of key components of ECM in the skeletal muscles of aged rats. Young (3 mo.) and older (21 mo.) adult male Wistar rats were submitted to a RT protocol (ladder climbing with 65, 85, 95, and 100% load), 3 times a week for 12 weeks. Forty-eight hours post-training, the soleus (SOL) and gastrocnemius (GAS) muscles were dissected for histological and mRNA analysis. RT mitigated the age-associated increase of connective tissue content in both muscles, even though mRNA levels of COL-1 and−3 were elevated in older trained rats. Overall, RT significantly elevated the gene expression of key components of connective tissue deposition (TGFβ and CTGF; MMP-2 and-9; TIMP-1 and−2) in the GAS and SOL muscles of older rats. In conclusion, RT blunted the age-induced accumulation of connective tissue concomitant to the upregulation of genes related to synthesis and degradation of the ECM network in the SOL and GAS muscles of older rats. Although our findings indicate that RT plays a crucial role reducing connective tissue accumulation in aged hindlimb muscles, key components of ECM turnover were paradoxically elevated. The phenotypic responses induced by RT were not accompanied by the gene expression of those components related to ECM turnover.
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影响因子：
2.7
作者：
Dheda, K;Huggett, JF;Zumla, A
通讯作者：
Zumla, A
影响因子：
2.8
作者：
GOLDSPINK, G;FERNANDES, K;WELLS, DJ
通讯作者：
WELLS, DJ
DOI：
10.6061/clinics/2014(04)08
发表时间：
2014
期刊：
Clinics (Sao Paulo, Brazil)
影响因子：
--
作者：
Souza MV;Leite RD;Souza Lino AD;Marqueti Rde C;Bernardes CF;Araújo HS;Bouskela E;Shiguemoto GE;Andrade Perez SE;Kraemer-Aguiar LG
通讯作者：
Kraemer-Aguiar LG
影响因子：
3.4
作者：
Gillies, Allison R.;Lieber, Richard L.
通讯作者：
Lieber, Richard L.
影响因子：
4.8
作者：
AIMES, RT;QUIGLEY, JP
通讯作者：
QUIGLEY, JP