Expression of the arsenite oxidation regulatory operon in Rhizobium sp. str. NT-26 is under the control of two promoters that respond to different environmental cues.
Expression of the arsenite oxidation regulatory operon in Rhizobium sp. str. NT-26 is under the control of two promoters that respond to different environmental cues.
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DOI：
10.1002/mbo3.567
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发表时间：
2018-06
期刊：
影响因子：
3.4
通讯作者：
Santini JM
中科院分区：
文献类型：
--
作者：
Corsini PM;Walker KT;Santini JM
根瘤菌NT-26是一种革兰氏阴性兼性化能自养亚砷酸盐氧化菌，已被用作研究亚砷酸盐氧化各个方面的模式生物，包括亚砷酸盐氧化的调节。当NT-26在存在或不存在亚砷酸盐的情况下生长时，三个调控基因aioX、aioS和aioR共转录。aioXSR操纵子在稳定期上调，但不通过生长培养基中亚砷酸盐的存在。确定了aioX上游的两个转录起始位点，从而鉴定了两个启动子：管家启动子RpoD和生长期依赖性启动子RpoE 2。启动子-lacZ融合证实了它们的组成型和稳定期表达。使用携带启动子-lacZ融合体和NT-26 rpoE 2基因的菌株，在缺乏rpoE 2同源物的大肠杆菌中体内证实了NT-26 σ因子RpoE 2参与作用于NT-26 RpoE 2启动子。使用计算机模拟方法来搜索其他RpoE 2启动子和AioR结合基序，并鉴定了可以由这些蛋白质调控的其他基因，包括参与群体感应，趋化性和运动性的基因，这些基因扩展了对亚砷酸盐的微生物代谢重要的信号网络。
Rhizobium sp. str. NT‐26 is a Gram‐negative facultative chemolithoautotrophic arsenite oxidizer that has been used as a model organism to study various aspects of arsenite oxidation including the regulation of arsenite oxidation. The three regulatory genes, aioX, aioS, and aioR, are cotranscribed when NT‐26 was grown in the presence or absence of arsenite. The aioXSR operon is upregulated in stationary phase but not by the presence of arsenite in the growth medium. The two transcription start sites upstream of aioX were determined which led to the identification of two promoters, the housekeeping promoter RpoD and the growth‐phase‐dependent promoter RpoE2. Promoter–lacZ fusions confirmed their constitutive and stationary phase expressions. The involvement of the NT‐26 sigma factor RpoE2 in acting on the NT‐26 RpoE2 promoter was confirmed in vivo in Escherichia coli, which lacks a rpoE2 homolog, using a strain carrying both the promoter–lacZ fusion and the NT‐26 rpoE2 gene. An in silico approach was used to search for other RpoE2 promoters and AioR‐binding motifs and led to the identification of other genes that could be regulated by these proteins including those involved in quorum sensing, chemotaxis, and motility expanding the signaling networks important for the microbial metabolism of arsenite.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
4.4
作者：
Moinier, Danielle;Slyemi, Djamila;Bonnefoy, Violaine
通讯作者：
Bonnefoy, Violaine
影响因子：
14.9
作者：
HARLEY, CB;REYNOLDS, RP
通讯作者：
REYNOLDS, RP
影响因子：
2.1
作者：
Sardiwal, Sunita;Santini, Joanne M.;Djordjevic, Snezana
通讯作者：
Djordjevic, Snezana
影响因子：
3.3
作者：
Andres J;Arsène-Ploetze F;Barbe V;Brochier-Armanet C;Cleiss-Arnold J;Coppée JY;Dillies MA;Geist L;Joublin A;Koechler S;Lassalle F;Marchal M;Médigue C;Muller D;Nesme X;Plewniak F;Proux C;Ramírez-Bahena MH;Schenowitz C;Sismeiro O;Vallenet D;Santini JM;Bertin PN
通讯作者：
Bertin PN